Dissecting the Genetic Basis of Extremely Large Grain Shape in Rice Cultivar ‘JZ1560’

Jie-Zheng Ying; Ji-Ping Gao; Jun-Xiang Shan; Mei-Zhen Zhu; Min Shi; Hong-Xuan Lin

doi:10.1016/j.jgg.2012.03.001

Volume 39 Issue 7

Jul. 2012

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Article Navigation > Journal of Genetics and Genomics > 2012 > 39(7): 325-333

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Dissecting the Genetic Basis of Extremely Large Grain Shape in Rice Cultivar ‘JZ1560’

doi: 10.1016/j.jgg.2012.03.001

a
National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
b
National Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

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Corresponding author: E-mail address: hxlin@sibs.ac.cn (Hong-Xuan Lin)
Received Date: 2011-12-10
Accepted Date: 2012-03-08
Rev Recd Date: 2012-03-08

Available Online: 2012-03-21

Publish Date: 2012-07-20

Abstract

Abstract

Rice grain shape, grain length (GL), width (GW), thickness (GT) and length-to-width ratio (LWR), are usually controlled by multiple quantitative trait locus (QTL). To elucidate the genetic basis of extremely large grain shape, QTL analysis was performed using an F₂ population derived from a cross between a japonica cultivar ‘JZ1560’ (extremely large grain) and a contrastingindica cultivar ‘FAZ1’ (small grain). A total number of 24 QTLs were detected on seven different chromosomes. QTLs for GL, GW, GT and LWR explained 11.6%, 95.62%, 91.5% and 89.9% of total phenotypic variation, respectively. Many QTLs pleiotropically controlled different grain traits, contributing complex traits correlation. GW2 and qSW5/GW5, which have been cloned previously to control GW, showed similar chromosomal locations with qGW2-1/qGT2-1/qLWR2-2 and qGW5-2/qLWR5-1 and should be the right candidate genes. Plants pyramiding GW2 and qSW5/GW5 showed a significant increase in GW compared with those carrying one of the two major QTLs. Furthermore, no significant QTL interaction was observed between GW2 and qSW5/GW5. These results suggested that GW2 and qSW5/GW5 might work in independent pathways to regulate grain traits. ‘JZ1560’ alleles underlying all QTLs contributed an increase in GW and GT and the accumulation of additive effects generates the extremely large grain shape in ‘JZ1560’.
- Rice,
- Quantitative trait locus,
- Additive effect,
- Grain shape

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References(34)

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