Deletion of the Initial 45 Residues of ARR18 Induces Cytokinin Response in Arabidopsis

Yan Liang; Xingchun Wang; Sulei Hong; Yansha Li; Jianru Zuo

doi:10.1016/j.jgg.2011.12.004

Volume 39 Issue 1

Jan. 2012

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Deletion of the Initial 45 Residues of ARR18 Induces Cytokinin Response in Arabidopsis

doi: 10.1016/j.jgg.2011.12.004

Yan Liang^a,
Xingchun Wang^{a, #},
Sulei Hong^{a, b},
Yansha Li^{a, b},
Jianru Zuo^a

a
State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b
Graduate School of Chinese Academy of Sciences, Beijing 100049, China

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Corresponding author: E-mail address: yliang@genetics.ac.cn (Yan Liang)
Received Date: 2011-10-08
Accepted Date: 2011-12-23
Rev Recd Date: 2011-12-22

Available Online: 2011-12-30

Publish Date: 2012-01-20

Abstract

Abstract

The plant hormone cytokinin plays important roles in various aspects of plant growth and development. Cytokinin signaling is mediated by a multistep phosphorelay similar to bacterial two-component system. Type-B ARRs lie at the end of the cytokinin signaling, typically mediating the output response. However, it is still unclear how type-B ARRs are regulated in response to cytokinin. Typical type-B ARR contains an N-terminal receiver domain and a C-terminal effector domain. In this study, we performed a genome-wild comparative analysis by overexpressing full length and C-terminal effector domain of seven representative type-B ARRs. Our results indicated that overexpression of C-terminal effector domain causes short primary roots and short hypocotyls without the addition of cytokinin, suggesting that the inhibitory role of the receiver domain in the activity of the effector domain is a common mechanism in type-B ARRs. To investigate how the receiver domain inhibits the activity of the effector domain, we performed a deletion analysis. We found that deletion of the initial 45 residues of ARR18 (the 45 residues from N-terminus) causes pleiotropic growth defects by directly inducing cytokinin responsive genes. Together, our results suggest that the initial 45 residues are critical for the inhibitory role of the receiver domain to the effector domain in ARR18.
- Cytokinin,
- Response regulator,
- ARR18,
- Receiver domain

Present address: College of Life Sciences, Shanxi Agricultural University, Taigu 030801, China.

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References(53)

References

[1]	Argueso, C.T., Raines, T., Kieber, J.J. Cytokinin signaling and transcriptional networks Curr. Opin. Plant Biol., 13 (2010),pp. 533-539
[2]	Argyros, R.D., Mathews, D.E., Chiang, Y.-H. et al. Plant Cell, 20 (2008),pp. 2102-2116
[3]	Baikalov, I., Schroder, I., Kaczor-Grzeskowiak, M. et al. Biochemistry, 35 (1996),pp. 11053-11061
[4]	Bechtold, N., Ellis, J., Pelletier, G. Arabidopsis Protoc., 82 (1993),pp. 259-266
[5]	Brandstatter, I., Kieber, J.J. Plant Cell, 10 (1998),pp. 1009-1019
[6]	D’Agostino, I.B., Deruere, J., Kieber, J.J. Plant Physiol., 124 (2000),pp. 1706-1717
[7]	D’Agostino, I.B., Kieber, J.J. Molecular mechanisms of cytokinin action Curr. Opin. Plant Biol., 2 (1999),pp. 359-364
[8]	Davies, P.J.
[9]	Dello Ioio, R., Linhares, F.S., Scacchi, E. et al. Curr. Biol., 17 (2007),pp. 678-682
[10]	Dello Ioio, R., Linhares, F.S., Sabatini, S. Emerging role of cytokinin as a regulator of cellular differentiation Curr. Opin. Plant Biol., 11 (2008),pp. 23-27
[11]	Dello Ioio, R., Nakamura, K., Moubayidin, L. et al. A genetic framework for the control of cell division and differentiation in the root meristem Science, 322 (2008),pp. 1380-1384
[12]	Desikan, R., Last, K., Harrett-Williams, R. et al. Plant J., 47 (2006),pp. 907-916
[13]	Ferreira, F.J., Kieber, J.J. Cytokinin signaling Curr. Opin. Plant Biol., 8 (2005),pp. 518-525
[14]	Gao, R., Stock, A.M. Biological insights from structures of two-component proteins Annu. Rev. Microbiol., 63 (2009),pp. 133-154
[15]	Gao, R., Stock, A.M. Molecular strategies for phosphorylation-mediated regulation of response regulator activity Curr. Opin. Microbiol., 13 (2010),pp. 160-167
[16]	Haberer, G., Kieber, J.J. Cytokinins. New insights into a classic phytohormone Plant Physiol., 128 (2002),pp. 354-362
[17]	Hass, C., Lohrmann, J., Albrecht, V. et al. EMBO J., 23 (2004),pp. 3290-3302
[18]	Heyl, A., Schmülling, T. Cytokinin signal perception and transduction Curr. Opin. Plant Biol., 6 (2003),pp. 480-488
[19]	Hosoda, K., Imamura, A., Katoh, E. et al. Plant Cell, 14 (2002),pp. 2015-2029
[20]	Huala, E., Stigter, J., Ausubel, F.M. J. Bacteriol., 174 (1992),pp. 1428-1431
[21]	Hwang, I., Sheen, J. Nature, 413 (2001),pp. 383-389
[22]	Hwang, I., Chen, H.-C., Sheen, J. Plant Physiol., 129 (2002),pp. 500-515
[23]	Imamura, A., Hanaki, N., Umeda, H. et al. Proc. Natl. Acad. Sci. USA, 95 (1998),pp. 2691-2696
[24]	Imamura, A., Hanaki, N., Nakamura, A. et al. Plant Cell. Physiol., 40 (1999),pp. 733-742
[25]	Imamura, A., Kiba, T., Tajima, Y. et al. Plant Cell. Physiol., 44 (2003),pp. 122-131
[26]	Ishida, K., Yamashino, T., Yokoyama, A. et al. Plant Cell. Physiol., 49 (2008),pp. 47-57
[27]	Kakimoto, T. Perception and signal transduction of cytokinins Annu. Rev. Plant Biol., 54 (2003),pp. 605-627
[28]	Kiba, T., Yamada, H., Sato, S. et al. Plant Cell. Physiol., 44 (2003),pp. 868-874
[29]	Kim, H.J., Ryu, H., Hong, S.H. et al. Proc. Natl. Acad. Sci. USA, 103 (2006),pp. 814-819
[30]	Lee, D.J., Park, J.Y., Ku, S.J. et al. Mol. Genet. Genomics, 277 (2007),pp. 115-137
[31]	Mason, M.G., Li, J., Mathews, D.E. et al. Plant Physiol., 135 (2004),pp. 927-937
[32]	Mok, D.W., Mok, M.C. Cytokinin metabolism and action Annu. Rev. Plant Physiol. Plant Mol. Biol., 52 (2001),pp. 89-118
[33]	Moubayidin, L., Perilli, S., Dello Ioio, R. et al. Curr. Biol., 20 (2010),pp. 1138-1143
[34]	Müller, B., Sheen, J. Advances in cytokinin signaling Science, 318 (2007),pp. 68-69
[35]	Müller, B., Sheen, J. Cytokinin and auxin interaction in root stem-cell specification during early embryogenesis Nature, 453 (2008),pp. 1094-1097
[36]	Nixon, B.T., Yennawar, H.P., Doucleff, M. et al. Biochemistry, 44 (2005),pp. 13962-13969
[37]	Nowak, E., Panjikar, S., Konarev, P. et al. J. Biol. Chem., 281 (2006),pp. 9659-9666
[38]	Osakabe, Y., Miyata, S., Urao, T. et al. Biochem. Biophys. Res. Commun., 293 (2002),pp. 806-815
[39]	Park, S., Meyer, M., Jones, A.D. et al. FASEB J., 16 (2002),pp. 1964-1966
[40]	Park, S., Zhang, H., Jones, A.D. et al. Biochemistry, 41 (2002),pp. 10934-10941
[41]	Rashotte, A.M., Carson, S.D., To, J.P. et al. Plant Physiol., 132 (2003),pp. 1998-2011
[42]	Ren, B., Liang, Y., Deng, Y. et al. Cell Res., 19 (2009),pp. 1178-1190
[43]	Robinson, V.L., Wu, T., Stock, A.M. Structural analysis of the domain interface in DrrB, a response regulator of the OmpR/PhoB subfamily J. Bacteriol., 185 (2003),pp. 4186-4194
[44]	Sakai, H., Aoyama, T., Oka, A. Plant J., 24 (2000),pp. 703-711
[45]	Sakai, H., Honma, T., Aoyama, T. et al. ARR1, a transcription factor for genes immediately responsive to cytokinins Science, 294 (2001),pp. 1519-1521
[46]	Stock, A.M., Robinson, V.L., Goudreau, P.N. Two-component signal transduction Annu. Rev. Biochem., 69 (2000),pp. 183-215
[47]	Tajima, Y., Imamura, A., Kiba, T. et al. Plant Cell Physiol., 45 (2004),pp. 28-39
[48]	To, J.P.C., Kieber, J.J. Cytokinin signaling: two-components and more Trend Plant Sci., 13 (2008),pp. 85-92
[49]	To, J.P.C., Haberer, G., Ferreira, F.J. et al. Plant Cell, 16 (2004),pp. 658-671
[50]	Werner, T., Schmulling, T. Cytokinin action in plant development Curr. Opin. Plant Biol., 12 (2009),pp. 527-538
[51]	West, A.H., Stock, A.M. Histidine kinases and response regulator proteins in two-component signaling systems Trends Biochem. Sci., 26 (2001),pp. 369-376
[52]	Yokoyama, A., Yamashino, T., Amano, Y.-I. et al. Plant Cell. Physiol., 48 (2007),pp. 84-96
[53]	Zuo, J., Niu, Q.W., Chua, N.H. An estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants Plant J., 24 (2000),pp. 265-273