MicroRNA-125b Induces Cancer Cell Apoptosis Through Suppression of Bcl-2 Expression

Aihua Zhao; Quan Zeng; Xiaoyan Xie; Junnian Zhou; Wen Yue; Yali Li; Xuetao Pei

doi:10.1016/j.jgg.2011.12.003

Volume 39 Issue 1

Jan. 2012

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Article Navigation > Journal of Genetics and Genomics > 2012 > 39(1): 29-35

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MicroRNA-125b Induces Cancer Cell Apoptosis Through Suppression of Bcl-2 Expression

doi: 10.1016/j.jgg.2011.12.003

Aihua Zhao^{a, b, c, #},
Quan Zeng^{b, #},
Xiaoyan Xie^b,
Junnian Zhou^b,
Wen Yue^b,
Yali Li^a,
Xuetao Pei^b

a
Department of Obstetrics and Gynecology, General Hospital of PLA, Beijing 100853, China
b
Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China
c
Department of Obstetrics and Gynecology, The 309th Hospital of PLA, Beijing 100091, China

More Information

Corresponding author: E-mail address: wenyue26@yahoo.com (Wen Yue); E-mail address: li-yali@hotmail.com (Yali Li); E-mail address: peixt@nic.bmi.ac.cn (Xuetao Pei)
Received Date: 2011-04-25
Accepted Date: 2011-12-23
Rev Recd Date: 2011-11-25

Available Online: 2011-12-28

Publish Date: 2012-01-20

Abstract

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatocellular carcinoma (HCC). We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3′UTR of Bcl-2 has binding sites for miR-125b. Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer cell apoptosis.
- miR-125b,
- Bcl-2,
- Apoptosis,
- Proliferation

These authors contributed equally to this work.

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References(20)

References

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