A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H2O2 production

Juan Luo; Li-Li Zhao; Si-Ying Gong; Xiang Sun; Peng Li; Li-Xia Qin; Ying Zhou; Wen-Liang Xu; Xue-Bao Li

doi:10.1016/j.jgg.2011.10.003

Volume 38 Issue 11

Nov. 2011

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Article Navigation > Journal of Genetics and Genomics > 2011 > 38(11): 557-565

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A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H2O2 production

doi: 10.1016/j.jgg.2011.10.003

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China

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Corresponding author: E-mail address: wenliangxu@yahoo.com.cn (Wen-Liang Xu); E-mail address: xbli@mail.ccnu.edu.cn (Xue-Bao Li)
Received Date: 2011-06-10
Accepted Date: 2011-10-18
Rev Recd Date: 2011-10-14

Available Online: 2011-10-28

Publish Date: 2011-11-20

Abstract

Abstract

The mitogen-activated protein kinase (MAPK) cascade is one of the major and evolutionally conserved signaling pathways and plays a pivotal role in the regulation of stress and developmental signals in plants. Here, we identified one gene, GhMPK6, encoding an MAPK protein in cotton. GFP fluorescence assay demonstrated that GhMAPK6 is a cytoplasm localized protein. Quantitative RT-PCR analysis revealed that mRNA accumulation of GhMPK6 was significantly promoted by abscisic acid (ABA). Overexpression of GhMPK6 gene in the T-DNA insertion mutant atmkk1 (SALK_015914) conferred a wild-type phenotype to the transgenic plants in response to ABA. Under ABA treatment, cotyledon greening/expansion in GhMPK6 transgenic lines and wild type was significantly inhibited, whereas the atmkk1 mutant showed a relatively high cotyledon greening/expansion ratio. Furthermore, CAT1 expression and H₂O₂ levels in leaves of GhMPK6 transgenic lines and wild type were remarkably higher than those of atmkk1 mutant with ABA treatment. Collectively, our results suggested that GhMPK6 may play an important role in ABA-induced CAT1 expression and H₂O₂ production.
- Mitogen-activated protein kinase (MAPK),
- Abscisic acid (ABA),
- Hypersensitivity

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