TSA1 interacts with CSN1/CSN and may be functionally involved in Arabidopsis seedling development in darkness

Wenjun Li; Baisheng Zang; Citao Liu; Lu Lu; Ning Wei; Kaiming Cao; Xing Wang Deng; Xiping Wang

doi:10.1016/j.jgg.2011.08.007

Volume 38 Issue 11

Nov. 2011

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TSA1 interacts with CSN1/CSN and may be functionally involved in Arabidopsis seedling development in darkness

doi: 10.1016/j.jgg.2011.08.007

a
State Key Laboratory of Genetic Engineering, Department of Biochemistry, Fudan University, Shanghai 200433, China
b
Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, Department of Biochemistry, College of Life Sciences, Peking University, Beijing 100871, China
c
Beijing Key Laboratory of Gene Engineering Drug and Biotechnology, Department of Biochemistry, College of Life Sciences, Beijing Normal University, Beijing 100875, China
d
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA

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Corresponding author: E-mail address: xipingwang@bnu.edu.cn (Xiping Wang)
Received Date: 2011-03-30
Accepted Date: 2011-08-04
Rev Recd Date: 2011-08-03

Available Online: 2011-09-21

Publish Date: 2011-11-20

Abstract

Abstract

The COP9 signalosome (CSN) is a multiprotein complex which participates in diverse cellular and developmental processes. CSN1, one of the subunits of CSN, is essential for assembly of the multiprotein complex via PCI (proteasome, COP9 signalosome and initiation factor 3) domain in the C-terminal half of CSN1. However, the role of the N-terminal domain (NTD) of CSN1, which is critical for the function of CSN, is not completely understood. Using a yeast two-hybrid (Y2H) screen, we found that the NTD of CSN1 interacts with TSK-associating protein 1 (TSA1), a reported Ca²⁺-binding protein. The interaction between CSN1 and TSA1 was confirmed by co-immunoprecipitation in Arabidopsis. tsa1 mutants exhibited a short hypocotyl phenotype in darkness but were similar to wild-type Arabidopsis under white light, which suggested that TSA1 might regulate Arabidopsis hypocotyl development in the dark. Furthermore, the expression of TSA1 was significantly lower in a csn1 null mutant (fus6), while CSN1 expression did not change in a tsa1 mutant with weak TSA1 expression. Together, these findings suggest a functional relationship between TSA1 and CSN1 in seedling development.
- CSN1,
- TSA1,
- Protein interaction,
- Hypocotyl

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References(44)

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