Development of upland rice introgression lines and identification of QTLs for basal root thickness under different water regimes

Junzhou Li; Deping Wang; Yan Xie; Hongliang Zhang; Guanglong Hu; Jinjie Li; Anyong Dai; Lifeng Liu; Zichao Li

doi:10.1016/j.jgg.2011.08.005

Volume 38 Issue 11

Nov. 2011

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Development of upland rice introgression lines and identification of QTLs for basal root thickness under different water regimes

doi: 10.1016/j.jgg.2011.08.005

a
Key Laboratory of Crop Heterosis and Utilization (MOE), and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
b
College of Agriculture, Henan Agricultural University, Zhengzhou 450002, China
c
China National Centre for Biotechnology Development, Beijing 100039, China

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Corresponding author: E-mail address: lizichao@cau.edu.cn (Zichao Li)
Received Date: 2011-02-13
Accepted Date: 2011-08-04
Rev Recd Date: 2011-08-01

Available Online: 2011-08-19

Publish Date: 2011-11-20

Abstract

Abstract

Introgression lines (ILs) are valuable materials for identifying quantitative trait loci (QTLs), evaluating genetic interactions, and marker assisted breeding. A set of 430 ILs (BC₅F₃) containing segments from upland tropical japonica cultivar IRAT109 in a lowland temperate japonica cultivar Yuefu background were developed. One hundred and seventy-six polymorphic markers were used to identify introgressed segments. No segment from IRAT109 was found in 160 lines. Introgressed segments of the other 270 lines covered 99.1% of the donor genome. The mean number of introgressed donor segments per individual was 3.3 with an average length of 14.4 cM. QTL analysis was conducted on basal root thickness (BRT) of the 270 ILs grown under irrigated lowland, upland and hydroponic conditions. A total of 22 QTLs affecting BRT were identified, six QTLs (qBRT3.1, qBRT3.2, qBRT6.1, qBRT8.2, qBRT9.1, and qBRT9.2) were consistently expressed under at least two environments (location and water regime), and qBRT7.2 was a new BRT QTL identified under lowland conditions. IL255 containing qBRT9.1 showed an increase of 10.09% and 7.07% BRT over cultivar Yuefu when grown under upland and lowland conditions, respectively. Using a population of 304 F_2:3 lines derived from the cross IL255×Yuefu, qBRT9.1 was validated and mapped to a 1.2 cM interval between RM24271 and RM566. The presence ofqBRT9.1 explained 12% of BRT variation. The results provide upland rice ILs and BRT QTLs for analyzing the genetic basis of drought resistance, detecting favorable genes from upland rice, and rice drought resistance breeding.
- Upland rice,
- Quantitative trait loci,
- Introgression lines,
- Basal root thickness

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References(52)

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