Association of WNK1 exon 1 polymorphisms with essential hypertension in Hani and Yi minorities of China

Yina Cun; Jin Li; Wenru Tang; Xiaozhi Sheng; Haijing Yu; Bingrong Zheng; Chunjie Xiao

doi:10.1016/j.jgg.2011.03.004

Volume 38 Issue 4

Apr. 2011

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2011 > 38(4): 165-171

PDF( 240 KB)

Association of WNK1 exon 1 polymorphisms with essential hypertension in Hani and Yi minorities of China

doi: 10.1016/j.jgg.2011.03.004

Laboratory for Conservation and Utilization of Bio-resources and Human Genetics, Center of Yunnan University, Kunming 650091, China

More Information

Corresponding author: E-mail address: cjxiao2009@gmail.com (Chunjie Xiao)
Received Date: 2010-10-18
Accepted Date: 2011-02-27
Rev Recd Date: 2011-01-19

Available Online: 2011-03-25

Publish Date: 2011-04-20

Abstract

Abstract

The association of polymorphisms in exon 1 of the WNK1 gene with essential hypertension in the minority groups of Hani and Yi of China was investigated in the case-control study. The sequence of 1257bp containing the WNK1 gene exon 1 was determined in 1307 individuals (649 essential hypertension subjects and 658 controls) to identify SNPs in Hani and Yi minority groups. Four of eleven previously known SNPs (rs3168640, rs11885, rs11554421 and rs34880640) were identified. The SNP analysis indicated that SNPs rs11885 and rs11554421 were significantly associated with hypertension in both Hani and Yi populations, and rs34880640 was significantly associated with hypertension in Hani but not in Yi population, adjusted for covariates. Haplotype analysis indicated that the haplotype H1 significantly decreased the risk of hypertension in both populations. These results suggested thatWNK1 polymorphisms were involved in the predisposition of essential hypertension in Hani and Yi populations and its effects showed a clear population specificity. This finding supported the importance of population specificity in determining the genetic factors associated with diseases and thus disease treatment.
- SNPs,
- Haplotype,
- Essential hypertension,
- Hani population,
- Yi population

These authors contribute equally to this work.

FullText(HTML)

References(29)

References

[1]	Barrett, J.C., Fry, B., Maller, J. et al. Haploview: analysis and visualization of LD and haplotype maps Bioinformatics, 21 (2005),pp. 263-265
[2]	Cope, G., Murthy, M., Golbang, A.P. et al. WNK1 affects surface expression of the ROMK potassium channel independent of WNK4 J. Am. Soc. Nephrol., 17 (2006),pp. 1867-1874
[3]	Delaloy, C., Lu, J., Houot, A.M. et al. Mol. Cell Biol., 23 (2003),pp. 9208-9221
[4]	Gao, L., Yang, Z.L., Yang, M.H. et al. A survey on prevalence of hypertension in Hani Tribe in Yunnan Province Chin. J. Hypertens. Res., 12 (2004),pp. 362-364
[5]	Golbang, A.P., Cope, G., Hamad, A. et al. Regulation of the expression of the Na/Cl cotransporter by WNK4 and WNK1: evidence that accelerated dynamin-dependent endocytosis is not involved Am. J. Physiol. Renal. Physiol., 291 (2006),pp. 1369-1376
[6]	Han, Y.F., Hui, R.T. Mol. Cardiol. Chin., 8 (2008),pp. 157-160
[7]	Kannel, W.B. Elevated systolic blood pressure as a cardiovascular risk factor Am. J. Cardiol., 85 (2000),pp. 251-255
[8]	Kokubo, Y., Kamide, K., Inamoto, N. et al. J. Hum. Genet., 49 (2004),pp. 507-515
[9]	Lander, E.S. The new genomics: global views of biology Science, 274 (1996),pp. 536-539
[10]	Lazrak, A., Liu, Z., Huang, C.L. Antagonistic regulation of ROMK by long and kidney-specific WNK1 isoforms Proc. Natl. Acad. Sci. USA, 103 (2006),pp. 1615-1620
[11]	Lifton, R.P., Gharavi, A.G., Geller, D.S. Molecular mechanisms of human hypertension Cell, 104 (2001),pp. 545-556
[12]	Lipworth, B., Koppelman, G.H., Wheatley, A.P. et al. Beta2 adrenoceptor promoter polymorphisms: extended haplotypes and functional effects in peripheral blood mononuclear cells Thorax, 57 (2002),pp. 61-66
[13]	Marteau, J.B., Zaiou, M., Siest, G. et al. Genetic determinants of blood pressure regulation J. Hypertens., 23 (2005),pp. 2127-2143
[14]	McGraw, D.W., Forbes, S.L., Kramer, L.A. et al. Polymorphisms of the 5’ leader cistron of the human beta2-adrenergic receptor regulate receptor expression J. Clin. Invest., 102 (1998),pp. 1927-1932
[15]	Mosterd, A., D’Agostino, R.B., Silbershatz, H. et al. Trends in the prevalence of hypertension, antihypertensive therapy, and left ventricular hypertrophy from 1950 to 1989 N. Engl. J. Med., 340 (1999),pp. 1221-1227
[16]	Naray-Fejes-Toth, A., Snyder, P.M., Fejes-Toth, G. Proc. Natl. Acad. Sci. USA, 101 (2004),pp. 17434-17439
[17]	Newhouse, S., Farrall, M., Wallace, C. et al. PLoS ONE, 4 (2009),p. e5003
[18]	Newhouse, S.J., Wallace, C., Dobson, R. et al. Hum. Mol. Genet., 14 (2005),pp. 1805-1814
[19]	Raffel, L.J. The epidemiology and genetic basis of common diseases Pediatr. Ann., 26 (1997),pp. 525-534
[20]	Scheet, P., Stephens, M. A fast and flexible statistical model for large-scale population genotype data: applications to inferring missing genotypes and haplotypic phase Am. J. Hum. Genet., 78 (2006),pp. 629-644
[21]	Shekarabi, M., Girard, N., Riviere, J.B. et al. J. Clin. Invest., 118 (2008),pp. 2496-2505
[22]	Subramanya, A.R., Yang, C.L., Zhu, X. et al. Dominant-negative regulation of WNK1 by its kidney-specific kinase-defective isoform Am. J. Physiol. Renal. Physiol., 290 (2006),pp. F619-F624
[23]	Tobin, M.D., Raleigh, S.M., Newhouse, S. et al. Circulation, 112 (2005),pp. 3423-3429
[24]	Turner, S.T., Schwartz, G.L., Chapman, A.B. et al. WNK1 kinase polymorphism and blood pressure response to a thiazide diuretic Hypertension, 46 (2005),pp. 758-765
[25]	Verissimo, F., Jordan, P. WNK kinases, a novel protein kinase subfamily in multi-cellular organisms Oncogene, 20 (2001),pp. 5562-5569
[26]	Wilson, F.H., Kahle, K.T., Sabath, E. et al. Proc. Natl. Acad. Sci. USA, 100 (2003),pp. 680-684
[27]	Wu, H.Y., Tang, W.R., Li, H.W. et al. Association of the beta2-adrenergic receptor gene with essential hypertension in the non-Han Chinese Yi minority human population J. Hypertens., 24 (2006),pp. 1041-1047
[28]	Yang, C.L., Angell, J., Mitchell, R. et al. WNK kinases regulate thiazide-sensitive Na–Cl cotransport J. Clin. Invest., 111 (2003),pp. 1039-1045
[29]	Zambrowicz, B.P., Abuin, A., Ramirez-Solis, R. et al. Wnk1 kinase deficiency lowers blood pressure in mice: a gene-trap screen to identify potential targets for therapeutic intervention Proc. Natl. Acad. Sci. USA, 100 (2003),pp. 14109-14114