Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process

Lianquan Zhang; Li Zhang; Jiangtao Luo; Wenjie Chen; Ming Hao; Baolong Liu; Zehong Yan; Bo Zhang; Huaigang Zhang; Youliang Zheng; Dengcai Liu; Yang Yen

doi:10.1016/j.jcg.2011.01.004

Volume 38 Issue 2

Feb. 2011

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Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process

doi: 10.1016/j.jcg.2011.01.004

a
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China
b
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
c
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA

More Information

Corresponding author: E-mail address: dcliu7@yahoo.com (Dengcai Liu); E-mail address: yang.yen@sdstate.edu (Yang Yen)
Received Date: 2010-08-24
Accepted Date: 2010-12-06
Rev Recd Date: 2010-11-11

Available Online: 2011-02-23

Publish Date: 2011-02-20

Abstract

Abstract

Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps: hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific F₁s. DHs produced in this way contain recombinant chromosomes in the genome(s) of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat (Triticum aestivum L.) and molecular markers were used to demonstrate the SynDH method.
- Allopolyploid,
- Doubled haploid,
- Unreduced gametes

These authors contributed equally to this work.

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References(36)

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