Overexpression of the tonoplast aquaporin AtTIP5;1 conferred tolerance to boron toxicity in Arabidopsis

Yongqi Pang; Lijuan Li; Fei Ren; Pingli Lu; Pengcheng Wei; Jinghui Cai; Lingguo Xin; Juan Zhang; Jia Chen; Xuechen Wang

doi:10.1016/S1673-8527(09)60057-6

Volume 37 Issue 6

Jun. 2010

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Article Navigation > Journal of Genetics and Genomics > 2010 > 37(6): 389-397

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Overexpression of the tonoplast aquaporin AtTIP5;1 conferred tolerance to boron toxicity in Arabidopsis

doi: 10.1016/S1673-8527(09)60057-6

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China

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Corresponding author: E-mail address: xcwang@cau.edu.cn (Xuechen Wang)
Received Date: 2010-01-09
Accepted Date: 2010-05-07
Rev Recd Date: 2010-04-29

Available Online: 2010-07-01

Publish Date: 2010-06-20

Abstract

Abstract

Boron (B) toxicity to plants is responsible for low crop productivity in many regions of the world. Here we report a novel and effective means to alleviate the B toxicity to plants under high B circumstance. Functional characterization of AtTIP5;1, an aquaporin gene, revealed that overexpression of AtTIP5;1 (OxAtTIP5;1) in Arabidopsis significantly increased its tolerance to high B toxicity. Compared to wild-type plants, OxAtTIP5;1 plants exhibited longer hypocotyls, accelerated development, increased silique production under high B treatments. GUS staining and quantitative RT-PCR (qRT-PCR) results demonstrated that the expression of AtTIP5;1 was induced by high B concentration treatment. Subcellular localization analysis revealed that the AtTIP5;1-GFP fusion protein was localized on the tonoplast membrane, which was consistent with the prediction based on bioinformatics. Taken together, our results suggest that AtTIP5;1 is involved in B transport pathway possibly via vacuolar compartmentation for B, and that overexpression of AtTIP5;1 in plants may provide an effective way to overcome the problem resulting from high B concentration toxicity.
- aquaporin,
- boron,
- toxicity tolerance

These authors contributed equally to this work.

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References

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