Methylation patterns in 5′ terminal regions of pluripotency-related genes in bovine in vitro fertilized and cloned embryos

Jie Lan; Song Hua; Hailin Zhang; Yongli Song; Jun Liu; Yong Zhang

doi:10.1016/S1673-8527(09)60047-3

Volume 37 Issue 5

May 2010

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2010 > 37(5): 297-304

PDF( 588 KB)

Methylation patterns in 5′ terminal regions of pluripotency-related genes in bovine in vitro fertilized and cloned embryos

doi: 10.1016/S1673-8527(09)60047-3

Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Institution of biotechnology, College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China

More Information

Corresponding author: E-mail address: zhangyong19561013@yahoo.cn (Yong Zhang)
Received Date: 2009-11-05
Accepted Date: 2010-02-10
Rev Recd Date: 2010-01-20

Available Online: 2010-05-31

Publish Date: 2010-05-20

Abstract

Abstract

In order to investigate DNA methylation profiles of five pluripotency-related genes (Oct4, Sox2, Nanog, Rex1 and Fgf4) during bovine maternal to zygotic transition (MZT) in both in vitro fertilized (IVF) and nuclear transfer (NT) embryos, sodium bisulfite sequencing method was used to detect DNA methylation levels, accompanied by the statistical analysis of embryo developmental rates. The results showed that Oct4, Nanog, Rex1 and Fgf4 were respectively demethylated by 25.22% (P < 0.01), 3.84% ( P > 0.05), 31.82% ( P < 0.01) and 10% ( P > 0.05) while Sox2 retained unmethylation during MZT in IVF embryos. By contrast, Oct4 and Rex1 respectively underwent demethylation by 23.04% (P < 0.01) and 6.02% ( P > 0.05), and, reversely, Sox2, Nanog and Fgf4 respectively experienced remethylation by 0.84% (P > 0.05), 5.39% ( P > 0.05) and 5.46% ( P > 0.05) during MZT in NT embryos. Interestingly, the CpG 14 site of Sox2 was specifically methylated in both 8-cell and morula NT embryos. In addition, the development of blastocysts between IVF and NT embryos showed no significant difference. DNA methylation analysis showed that only Oct4 and Sox2 underwent the correct methylation reprogramming process, which may be responsible for the development of blastocysts of NT embryos to a certain extent. In conclusion, the five genes respectively experienced demethylation to different extents and incomplete DNA methylation reprogramming during bovine MZT in both IVF and NT embryos, suggesting that they may be used as indicators for bovine embryo developmental competence.
- bovine,
- DNA methylation,
- Fgf4,
- Nanog,
- Oct4,
- Rex1,
- Sox2

FullText(HTML)

References(29)

References

[1]	Babaie, Y., Herwig, R., Greber, B. et al. Stem Cells, 25 (2007),pp. 500-510
[2]	Blelloch, R., Wang, Z.D., Meissner, A. et al. Reprogramming efficiency following somatic cell nuclear transfer is influenced by the differentiation and methylation state of the donor nucleus Stem Cells, 24 (2006),pp. 2007-2013
[3]	Boulet, A.M., Capecchi, M.R. Dev. Biol., 319 (2008),p. 509
[4]	Brevini, T.A.L., Antonini, S., Cillo, F. et al. Reprod. Fert. Develop., 20 (2008),p. 165
[5]	Chew, J.L., Loh, Y.H., Zhang, W.S. et al. Mol. Cell. Biol., 25 (2005),pp. 6031-6046
[6]	Dean, W., Santos, F., Reik, W. Epigenetic reprogramming in early mammalian development and following somatic nuclear transfer Semin. Cell Dev. Biol., 14 (2003),pp. 93-100
[7]	Dean, W., Santos, F., Stojkovic, M. et al. Conservation of methylation reprogramming in mammalian development: aberrant reprogramming in cloned embryos Proc. Natl. Acad. Sci. USA, 98 (2001),pp. 13734-13738
[8]	Degrelle, S.A., Campion, E., Cabau, C. et al. Molecular evidence for a critical period in mural trophoblast development in bovine blastocysts Dev. Biol., 288 (2005),pp. 448-460
[9]	Giraldo, A.A., Hylan, D.A., Ballard, C.B. et al. Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos Biol. Reprod., 78 (2008),pp. 832-840
[10]	Hohn, T., Corsten, S., Rieke, S. et al. Methylation of coding region alone inhibits gene expression in plant protoplasts Proc. Natl. Acad. Sci. USA, 93 (1996),pp. 8334-8339
[11]	Hua, S., Zhang, Y., Song, K. et al. Development of bovine-ovine interspecies cloned embryos and mitochondria segregation in blastomeres during preimplantation Anim. Reprod. Sci., 105 (2008),pp. 245-257
[12]	Illingworth, R.S., Bird, A.P. CpG islands – ‘A rough guide’ FEBS Lett., 583 (2009),pp. 1713-1720
[13]	Kang, Y.K., Koo, D.B., Park, J.S. et al. Aberrant methylation of donor genome in cloned bovine embryos Nat. Genet., 28 (2001),pp. 173-177
[14]	Kim, J.W., Chu, J.L., Shen, X.H. et al. An extended transcriptional network for pluripotency of embryonic stem cells Cell, 132 (2008),pp. 1049-1061
[15]	Kosaka, N., Sakamoto, H., Terada, M. et al. Dev. Dynam., 238 (2009),pp. 265-276
[16]	Kurosaka, S., Eckardt, S., McLaughlin, K.J. Biol. Reprod., 71 (2004),pp. 1578-1582
[17]	Lawrence, R.J., Earley, K., Pontes, O. et al. A concerted DNA methylation/histone methylation switch regulates rRNA gene dosage control and nucleolar dominance Mol. Cell, 13 (2004),pp. 599-609
[18]	Lin, L., Li, Q., Zhang, L. et al. Aberrant epigenetic changes and gene expression in cloned cattle dying around birth BMC Dev. Biol., 8 (2008),pp. 1-10
[19]	Meirelles, F., Caetano, A.R., Watanabe, Y.F. et al. Genome activation and developmental block in bovine embryos Anim. Reprod. Sci., 82–83 (2004),pp. 13-20
[20]	Pesce, M., Scholer, H.R. Stem Cells, 19 (2001),pp. 271-278
[21]	Polejaeva, I.A., Chen, S.H., Vaught, T.D. et al. Cloned pigs produced by nuclear transfer from adult somatic cells Nature, 407 (2000),pp. 86-90
[22]	Reik, W., Dean, W., Walter, J. Epigenetic reprogramming in mammalian development Science, 293 (2001),pp. 1089-1093
[23]	Rodda, D.J., Chew, J.L., Lim, L.H. et al. J. Biol. Chem., 280 (2005),pp. 24731-24737
[24]	Rogers, M.B., Hosler, B.A., Gudas, L.J. Development, 113 (1991),pp. 815-824
[25]	Rountree, M.R., Selker, E.U. Gene. Dev., 11 (1997),pp. 2383-2395
[26]	Shi, W., Haaf, T. Aberrant methylation patterns at the two-cell stage as an indicator of early developmental failure Mol. Reprod. Dev., 63 (2002),pp. 329-334
[27]	Tachibana, M., Clepper, L., Michelle, S. et al. Bio. Reprod., 81 (2009),p. 248
[28]	Wilmut, I., Schnieke, A.E., McWhir, J. et al. Viable offspring derived from fetal and adult mammalian cells Nature, 385 (1997),pp. 810-813
[29]	Wrenzycki, C., Herrmann, D., Keskintepe, L. et al. Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos Hum. Reprod., 16 (2001),pp. 893-901