The Post-meiotic Deficicent Anther1 (PDA1) gene is required for post-meiotic anther development in rice

Lifang Hu; Hexin Tan; Wanqi Liang; Dabing Zhang

doi:10.1016/S1673-8527(09)60023-0

Volume 37 Issue 1

Jan. 2010

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2010 > 37(1): 37-46

PDF( 1405 KB)

The Post-meiotic Deficicent Anther1 (PDA1) gene is required for post-meiotic anther development in rice

doi: 10.1016/S1673-8527(09)60023-0

a
School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
b
Bio-X Research Center, Key Laboratory of Genetics & Development and Neuropsychiatric Diseases, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200240, China

More Information

Corresponding author: E-mail address: zhangdb@sjtu.edu.cn (Dabing Zhang)
Received Date: 2009-09-24
Accepted Date: 2009-12-02
Rev Recd Date: 2009-10-26

Available Online: 2010-02-18

Publish Date: 2010-01-20

Abstract

Abstract

To understand the molecular mechanism of male reproductive development in the model crop rice, we isolated a complete male sterile mutant post-meiotic deficient anther1 (pda1) from a γ-ray-treated rice mutant library. Genetic analysis revealed that the pda1 mutant was controlled by a recessive nucleus gene. The pda1 mutant anther seemed smaller with white appearance. Histological analysis demonstrated that the pda1 mutant anther undergoes normal early tapetum development without obvious altered meiosis. However, the pda1 mutant displayed obvious defects in postmeiotic tapetal development, abnormal degeneration occurred in the tapetal cells at stage 9 of anther development. Also we observed abnormal lipidic Ubisch bodies from the tapetal layer of the pda1 mutant, causing no obvious pollen exine formation. RT-PCR analysis indicated that the expression of genes involved in anther development including GAMYB, OsC4 and Wax-deficient anther1 (WDA1) was greatly reduced in the pda1 mutant anther. Using map-based cloning approach, the PDA1 gene was finely mapped between two markers HLF610 and HLF627 on chromosome 6 using 3,883 individuals of F₂ population. The physical distance between HLF610 and HLF627 was about 194 kb. This work suggests that PDA1 is required for post-meiotic tapetal development and pollen/microspore formation in rice.
- rice,
- anther,
- degeneration,
- pollen exine,
- mapping

FullText(HTML)

References(38)

References

[1]	Albrecht, C., Russinova, E., Hecht, V. et al. Plant Cell, 17 (2005),pp. 3337-3349
[2]	Aya, K., Ueguchi-Tanaka, M., Kondo, M. et al. Plant Cell, 21 (2009),pp. 1453-1472
[3]	Canales, C., Bhatt, A.M., Scott, R. et al. Curr. Biol., 12 (2002),pp. 1718-1727
[4]	Chaudhury, A.M. Nuclear genes controlling male fertility Plant Cell, 5 (1993),pp. 1277-1283
[5]	Chen, L., Chu, H.W., Yuan, Z. et al. Journal of Xiamen University (Natural Science), 45 (2006),pp. 82-85
[6]	Feng, J.H., Lu, Y.G., Liu, X.D. et al. Chinese J. Rice Sci., 15 (2001),pp. 21-28
[7]	Goldberg, R.B., Beals, T.P., Sanders, P.M. Anther development: basic principles and practical applications Plant Cell, 5 (1993),pp. 1217-1229
[8]	Han, M.J., Jung, K.H., Yi, G. et al. Plant Cell Physiol., 47 (2006),pp. 1457-1472
[9]	Ito, T., Shinozaki, K. Plant Cell Physiol., 43 (2002),pp. 1285-1292
[10]	Itoh, J., Nonomura, K., Ikeda, K. et al. Rice plant development: from zygote to spikelet Plant Cell Physiol., 46 (2005),pp. 23-47
[11]	Jung, K.H., An, G., Ronald, P.C. Towards a better bowl of of rice: assigning function to tens of thousands of rice genes Nat. Rev. Genet., 9 (2008),pp. 91-101
[12]	Jung, K.H., Han, M.J., Lee, D.Y. et al. Plant Cell, 18 (2006),pp. 3015-3032
[13]	Jung, K.H., Han, M.J., Lee, Y.S. et al. Plant Cell, 17 (2005),pp. 2705-2722
[14]	Kaneko, M., Inukai, Y., Ueguchi-Tanaka, M. et al. Plant Cell, 16 (2004),pp. 33-44
[15]	Lee, S., Jung, K.H., An, G. et al. Plant Mol. Biol., 54 (2004),pp. 755-765
[16]	Li, N., Zhang, D.S., Liu, H.S. et al. Plant Cell, 18 (2006),pp. 2999-3014
[17]	Liu, Y., Yao, Z.X., Bendavid, C. et al. Haploinsufficiency of cytochrome P450 17alpha-hydroxylase/17,20 lyase (CYP17) causes infertility in male mice Mol. Endocrinol., 19 (2005),pp. 2380-2389
[18]	Ma, H. Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants Annu. Rev. Plant Biol., 56 (2005),pp. 393-434
[19]	Murray, M.G., Thompson, W.F. Rapid isolation of high molecular weight plant DNA Nucleic. Acids Res., 8 (1980),pp. 4321-4325
[20]	Nonomura, K., Miyoshi, K., Eiguchi, M. et al. Plant Cell, 15 (2003),pp. 1728-1739
[21]	Nonomura, K., Nakano, M., Fukuda, T. et al. Plant Cell, 16 (2004),pp. 1008-1020
[22]	Nonomura, K.I., Nakano, M., Murata, K. et al. Mol. Genet. Genomics, 271 (2004),pp. 121-129
[23]	Shen, Y.J., Jiang, H., Jin, J.P. et al. Development of genome-wide DNA polymorphism database for map-based cloning of rice genes Plant Physiol., 135 (2004),pp. 1198-1205
[24]	Sorensen, A.M., Krober, S., Unte, U.S. et al. Plant J., 33 (2003),pp. 413-423
[25]	Suwabe, K., Suzuki, G., Takahashi, H. et al. Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray Plant Cell Physiol., 49 (2008),pp. 1407-1416
[26]	Tsuchiya, T., Toriyama, K., Ejiri, S. et al. Molecular characterization of rice genes specifically expressed in the anther tapetum Plant Mol. Biol., 26 (1994),pp. 1737-1746
[27]	Tsuchiya, T., Toriyama, K., Nasrallah, M.E. et al. Isolation of genes abundantly expressed in rice anthers at the microspore stage Plant Mol. Biol., 20 (1992),pp. 1189-1193
[28]	Tsuji, H., Aya, K., Ueguchi-Tanaka, M. et al. Plant J., 47 (2006),pp. 427-444
[29]	Wang, Y., Wang, Y.F., Zhang, D.B. J. Plant Physiol. Mol. Biol., 32 (2006),pp. 527-534
[30]	Wilson, Z.A., Morroll, S.M., Dawson, J. et al. Plant J., 28 (2001),pp. 27-39
[31]	Wilson, Z.A., Zhang, D.B. J. Exp. Bot., 60 (2009),pp. 1479-1492
[32]	Yang, C., Vizcay-Barrena, G., Conner, K. et al. MALE STERILITY1 is required for tapetal development and pollen wall biosynthesis Plant Cell, 19 (2007),pp. 3530-3548
[33]	Yang, S.L., Xie, L.F., Mao, H.Z. et al. Plant Cell, 15 (2003),pp. 2792-2804
[34]	Zhang, D.S., Liang, W.Q., Yuan, Z. et al. Mol. Plant, 1 (2008),pp. 599-610
[35]	Zhang, D.B., Wilson, Z.A. Stamen specification and anther development in rice Chinese Sci. Bull., 54 (2009),pp. 2342-2353
[36]	Zhang, W., Sun, Y., Timofejeva, L. et al. Development, 133 (2006),pp. 3085-3095
[37]	Zhang, Z.B., Zhu, J., Gao, J.F. et al. Plant J., 52 (2007),pp. 528-538
[38]	Zhao, D.Z., Wang, G.F., Speal, B. et al. Genes Dev., 16 (2002),pp. 2021-2031