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Volume 35 Issue 12
Dec.  2008
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Article Navigation >  Journal of Genetics and Genomics  > 2008  >  35(12): 701-714

Progress in gene transfer by germ cells in mammals

doi: 10.1016/S1673-8527(08)60225-8
  • a 

    Laboratory Animal Center, Peking University People's Hospital, Beijing 100044, China

  • b 

    School of Basic Medical Sciences, Peking University, Beijing 100191, China

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  • Corresponding author: E-mail address: niuyd07@yahoo.com.cn (Yidong Niu)
  • Received Date: 2008-07-19
  • Accepted Date: 2008-10-30
  • Rev Recd Date: 2008-10-21
    • Available Online: 2008-12-23
  • Publish Date: 2008-12-20
    Abstract
  • Use of germ cells as vectors for transgenesis in mammals has been well developed and offers exciting prospects for experimental and applied biology, agricultural and medical sciences. Such approach is referred to as either male germ cell mediated gene transfer (MGCMGT) or female germ cell mediated gene transfer (FGCMGT) technique. Sperm-mediated gene transfer (SMGT), including its alternative method, testis-mediated gene transfer (TMGT), becomes an established and reliable method for transgenesis. They have been extensively used for producing transgenic animals. The newly developed approach of FGCMGT, ovary-mediated gene transfer (OMGT) is also a novel and useful tool for efficient transgenesis. This review highlights an overview of the recent progress in germ cell mediated gene transfer techniques, methods developed and mechanisms of nucleic acid uptake by germ cells.
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