Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)

Qiushi Wang; Xianchun Sang; Yinghua Ling; Fangming Zhao; Zhenglin Yang; Yunfeng Li; Guanghua He

doi:10.1016/S1673-8527(08)60160-5

Volume 36 Issue 11

Nov. 2009

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2009 > 36(11): 679-684

PDF( 323 KB)

Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)

doi: 10.1016/S1673-8527(08)60160-5

Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture; Rice Research Institute, Southwest University, Chongqing 400716, China

More Information

Corresponding author: E-mail address: hegh1968@yahoo.com.cn (Guanghua He)
Received Date: 2009-06-26
Accepted Date: 2009-09-23
Rev Recd Date: 2009-08-23

Available Online: 2009-11-20

Publish Date: 2009-11-20

Abstract

Abstract

A novel zebra mutant, zebra-15, derived from the restorer line Jinhui10 (Oryza sativa L. ssp. indica) treated by EMS, displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased (55.4%) and the ratio of Chla/Chlb increased (90.2%) significantly in the yellow part of the zebra-15, compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F₂ segregation populations derived from the cross of Xinong1A and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the Xinong1A and the zebra-15, their F₂ population was then used for gene mapping. Zebra-15 (Z-15) gene was primarily restricted on the short arm of chromosome 5 by 150 F₂ recessive individuals, 19.6 cM from marker RM3322 and 6.0 cM from marker RM6082. Thirty-six SSR markers were newly designed in the restricted location, and the Z-15 was finally located between markers nSSR516 and nSSR502 with the physical region 258 kb by using 1,054 F₂ recessive individuals.
- molecular mapping,
- zebra mutant,
- chlorophyll

FullText(HTML)

References(34)

References

[1]	Abenes, M.L.P., Tabien, R.E., McCouch, S.R. et al. Orientation and integration of the classical and molecular genetic maps of chromosome 11 in rice Euphytica, 76 (1994),pp. 81-87
[2]	Alberte, R.S., Hesketh, J.D., Hofstra, G. et al. Composition and activity of the photosynthetic apparatus in temperature sensitive mutants of higher plants Proc. Natl. Acad. Sci. USA, 71 (1974),pp. 2414-2418
[3]	Chen, G., Bi, Y.R., Li, N. Plant J., 41 (2005),pp. 364-375
[4]	Chen, T., Zhang, Y.D., Zhao, L. et al. Physiological character and gene mapping in a new green-revertible albino mutant in rice J. Genet. Genomics, 34 (2007),pp. 331-338
[5]	Eckhardt, U., Grimm, B., Hörtensteiner, S. Recent advances in chlorophyll biosynthesis and breakdown in higher plants Plant Mol. Biol., 56 (2004),pp. 1-14
[6]	Gálová, E., Bohmová, B., SěvSěoviSěová, A. Analysis of some barley chlorophyll mutants and their response to temperature stress Photosynthetica, 38 (2000),pp. 29-35
[7]	He, R.F., Ding, Y., Yu, J.H. et al. The changes of chlorophyll content and several enzyme activities in zebra-leaf rice Journal of Wuhan University (Natural Sciences Edition), 46 (2000),pp. 761-765
[8]	Hirochika, H., Guiderdoni, E., An, G. et al. Rice mutant resources for gene discovery Plant Mol. Biol., 54 (2004),pp. 325-334
[9]	Huang, X.Q., Zhao, H.X., Dong, C.L. et al. Chlorophyll-deficit rice mutants and their research advances in Biology Acta Botanica Boreali-Occidentalia Sinica, 25 (2005),pp. 1685-1691
[10]	Iwata, N., Omura, T., Satoh, H. Linkage studies in rice. The sequence of genes at the eighth and eleventh linkage groups Japan J. Breed, 28 (1978),pp. 170-171
[11]	Iwata, N., Satoh, H., Omura, T. The relationships between chromosomes identified cytologically and linkage groups Rice Genet. Newsl., 1 (1984),pp. 128-132
[12]	Kosambi, D.D. The estimation of map distances from recombination values Ann. Eugen., 12 (1944),pp. 172-175
[13]	Lander, E.S., Green, P., Abrahamson, J. et al. Mapmarker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations Genomics, 1 (1987),pp. 174-181
[14]	Lichtenthaler, H.K. Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes Method Enzymol., 48 (1987),pp. 350-382
[15]	Liu, W.Z., Fu, Y.P., Hu, G.C. et al. Planta, 226 (2007),pp. 785-795
[16]	Luo, Z.K., Yang, Z.L., Zhong, B.Q. et al. Genome, 50 (2007),pp. 811-817
[17]	Markwell, J., Osterman, J.C. Plant Physiol., 98 (1992),pp. 392-394
[18]	Mochizuki, N., Brusslan, J.A., Larkin, R. et al. Proc. Natl. Acad. Sci. USA, 98 (2001),pp. 2053-2058
[19]	Murray, M.G., Thompson, W.F. Rapid isolation of high molecular weight plant DNA Nucl. Acids Res., 8 (1980),pp. 4321-4325
[20]	Panaud, O., Chen, X., McCouch, S.R. Mol. Gen. Genet., 259 (1996),pp. 597-607
[21]	Pasini, L., Bruschini, S., Bertoli, A. et al. Photosynthetic performance of cold-sensitive mutants of maize at low temperature Physiol. Plant, 124 (2005),pp. 362-370
[22]	Rüdiger, W. Chlorophyll metabolism: From outer space down to the molecular level Phytochemistry, 46 (1997),pp. 1151-1167
[23]	Sanchez, A.C., Khush, G.S. Seven new genes for zebra character in rice Rice Genetics Newsletter, 9 (1992),pp. 68-71
[24]	Sanchez, A.C., Khush, G.S. Chromosomal location of some marker genes in rice using the primary trisomics J. Hered., 85 (1994),pp. 297-300
[25]	Sanchez, A.C., Khush, G.S. L. SABRAO J. Breeding Genet., 30 (1998),pp. 51-60
[26]	Sang, X.C., Yang, Z.L., Zhong, B.Q. et al. Assessment of purity of rice CMS lines using cpDNA marker Euphytica, 152 (2006),pp. 177-183
[27]	Shao, J.R., Sun, J.S., Xie, R. Journal of Leshan Teachers College, 18 (2003),pp. 50-53
[28]	Shao, J.R., Tang, M., Xie, R. Relation ship between the change of protein and the content of chlorophyll in the leaves of temperature-sensitive chlorophyll mutant 1103s of rice during the period of appearing chequered with green and chlorosis Southwest China Journal of Agricultural Sciences, 11 (1998),pp. 14-19
[29]	Shao, J.R., Wang, Y.Z., Liu, Y.S. et al. Acta Botanica Sinica, 41 (1999),pp. 20-24
[30]	Shao, J.R., Zhou, S.C., Xie, R. Journal of Sichuan University (Natural Science Edition), 34 (1997),pp. 688-692
[31]	Sugimoto, H., Kusumi, K., Tozawa, Y. et al. The virescent-2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation Plant Cell Physiol., 45 (2004),pp. 985-996
[32]	Wu, Z.M., Zhang, X., He, B. et al. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis Plant Physiol., 145 (2007),pp. 29-40
[33]	Xie, R., Zeng, Z.M., Liu, C.Y. et al. Characteristics analysis of reverse mutation on green-yellow band trait in rice Southwest China Journal of Agricultural Sciences, 20 (2007),pp. 1-5
[34]	Yoshimura, A., Ideta, O., Iwata, N. Linkage map of phenotype and RFLP markers in rice Plant Mol. Biol., 35 (1997),pp. 49-60