The construction of a genetic linkage map of non-heading Chinesecabbage (Brassica campestris ssp. chinensis Makino)

Yan Cheng; Jianfeng Geng; Jingyi Zhang; Qian Wang; Qingyu Ban; Xilin Hou

doi:10.1016/S1673-8527(08)60140-X

Volume 36 Issue 8

Aug. 2009

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2009 > 36(8): 501-508

PDF( 599 KB)

The construction of a genetic linkage map of non-heading Chinesecabbage (Brassica campestris ssp. chinensis Makino)

doi: 10.1016/S1673-8527(08)60140-X

a
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
b
Key laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
c
Institute of Horticulture, Henan Academy of Agricultural Science, Zhengzhou, 450002, China

More Information

Corresponding author: E-mail address: hxl@njau.edu.cn (Xilin Hou)
Received Date: 2008-11-09
Accepted Date: 2009-03-13
Rev Recd Date: 2009-03-01

Available Online: 2009-08-14

Publish Date: 2009-08-20

Abstract

Abstract

Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is one of the most important vegetables in eastern China. A genetic linkage map was constructed using 127 doubled haploid (DH) lines, and the DH population was derived from a commercial hybrid “Hanxiao” (lines SW-13 × L-118). Out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups(LGs). Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios, and the direction ofdistorted segregation was mainly toward the paternal parent L-118. After sequentially removing the markers that had an interval distancesmaller than 1 cM from the upper marker, the overall quality of the linkage map was increased. Two hundred and sixty-eight molecularmarkers were mapped into 10 LGs, which were anchored to the corresponding chromosome of theB. rapa reference map based on common simple sequence repeat (SSR) markers. The map covers 973.38 cM of the genome and the average interval distance between markerswas 3.63 cM. The number of markers on each LG ranged from 18 (R08) to 64 (R07), with an average interval distance within a single LGfrom 1.70 cM (R07) to 6.71 cM (R06). Among these mapped markers, 169 were sequence-related amplified polymorphism (SRAP) molecular markers, 50 were SSR markers and 49 were random amplification polymorphic DNA (RAPD) markers. With further saturation tothe LG, the current map offers a genetic tool for loci analysis for important agronomic traits.
- non-heading Chinese cabbage,
- genetic linkage map,
- SRAP,
- SSR,
- RAPD

FullText(HTML)

References(40)

References

[1]	Armstrong, K.C., Keller, W.A. Can. J. Genet. Cytol., 24 (1982),pp. 735-739
[2]	Beavis, W.D., Grant, D. Theor. Appl. Genet., 82 (1991),pp. 636-644
[3]	Choi, S.R., Teakle, G.R., Plaha, P. et al. Theor. Appl. Genet., 115 (2007),pp. 777-792
[4]	Chuong, P.V., Deslauriesrs, C., Kott, L.S. et al. Can. J. Bot., 66 (1988),pp. 1653-1657
[5]	Chyi, Y.-S., Hoeneche, M.E., Sernyk, J.L. Genome, 35 (1992),pp. 746-757
[6]	Cloutier, S., Cappadocia, M., Landry, B.S. Theor. Appl. Genet., 91 (1995),pp. 841-847
[7]	Cui, X.M., Dong, Y.X., Hou, X.L. et al. Agr. Sci. Chin., 7 (2008),pp. 257-265
[8]	Ferreira, M.E., Williams, P.H., Osborn, T.C. Theor. Appl. Genet., 89 (1994),pp. 615-621
[9]	Ferriol, M., Picó, B., Nuez, F. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers Theor. Appl. Genet., 107 (2003),pp. 271-282
[10]	Foisset, N., Delourme, R., Barret, P. et al. Theor. Appl. Genet., 93 (1996),pp. 1017-1025
[11]	Geng, J.F., Zhu, C.S., Zhang, X.W. et al. A genetic linkage map of nonheading Chinese cabbage J. Am. Soc. Hort. Sci., 132 (2007),pp. 816-823
[12]	Gosselin, I., Zhou, Y., Bousquet, J. et al. Theor. Appl. Genet., 104 (2002),pp. 987-997
[13]	Hamwieh, A., Udupa, S.M., Choumane, W. et al. Theor. Appl. Genet., 110 (2005),pp. 669-677
[14]	Kasha, K.J., Kao, K.N. Nature, 225 (1970),pp. 874-876
[15]	Kosambi, D.D. The estimation of map distance from recombination values Ann. Eugen., 12 (1944),pp. 172-175
[16]	Kim, J.S., Chung, T.Y., King, G.J. et al. Genetics, 174 (2006),pp. 29-39
[17]	Kole, C., Kole, P., Vogelzang, R. et al. J. Hered., 88 (1997),pp. 553-557
[18]	Li, G., Quiros, C.F. Sequence related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: Its application to mapping and gene tagging in Brassica Theor. Appl. Genet., 103 (2001),pp. 455-461
[19]	Liu, R.H., Meng, J.L. MapDraw: A microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data Hereditas, 25 (2003),pp. 317-321
[20]	Lorieux, M., Perrier, X., Goffinet, B. et al. Theor. Appl. Genet., 90 (1995),pp. 81-89
[21]	Lorieux, M.B., Perrier, G.X., González de Léon, D. et al. Maximum likelihood models for mapping genetic markers showing segregation distortion. 1. Backcross population Theor. Appl. Genet., 90 (1995),pp. 73-80
[22]	Lowe, A.J., Moule, C., Trick, M. et al. Theor. Appl. Genet., 108 (2004),pp. 1103-1112
[23]	Nag, D.K., White, M.A., Petes, T.D. Palindromic sequences in heteroduplex DNA inhibit mismatch repair in yeast Nature, 340 (1989),pp. 318-320
[24]	Nicolas, A., Rossignol, J.L. Gene conversion: Point mutation heterozygosities lower heteroduplex formation EMBO J., 2 (1983),pp. 2265-2270
[25]	Nozaki, T., Kunazaki, A., Koba, T. et al. Euphytica, 95 (1997),pp. 115-123
[26]	Pradhan, A.K., Gupta, V., Mukhopadhyay, A. et al. Theor. Appl. Genet., 106 (2003),pp. 607-614
[27]	Qi, X., Stam, P., Lindhout, P. Comparison and integration of four barley genetic maps Genome, 39 (1996),pp. 379-394
[28]	Sakamoto, K., Saito, A., Hayashida, N. et al. Theor. Appl. Genet., 117 (2008),pp. 759-767
[29]	Schranz, M.E., Lysak, M.A., Mitchell-Olds, T.M. The ABC's of comparative genomics in the Brassicaceae: Building blocks of crucifer genomes Trends Plant Sci., 11 (2006),pp. 535-542
[30]	Soengas, P., Hand, P., Vicente, J.G. et al. Theor. Appl. Genet., 114 (2007),pp. 637-645
[31]	Song, K.M., Suzuki, J.Y., Slocum, M.K. et al. Theor. Appl. Genet., 82 (1991),pp. 296-304
[32]	Suwabe, K., Iketani, H., Nunome, T. et al. Theor. Appl. Genet., 104 (2002),pp. 1092-1098
[33]	Suwabe, K., Iketani, H., Nunome, T. et al. Breed Res., 54 (2004),pp. 85-90
[34]	Suwabe, K., Tsukazaki, H., Iketani, H. et al. Genetics, 173 (2006),pp. 309-319
[35]	Takahate, Y., Keller, W.A. Plant Sci., 74 (1991),pp. 235-242
[36]	Teutonico, R.A., Osborn, T.C. Theor. Appl. Genet., 89 (1994),pp. 885-894
[37]	Uzunova, M., Ecke, W., Weissleder, K. et al. Theor. Appl. Genet., 90 (1995),pp. 194-204
[38]	Wu, J., Yuan, Y.X., Zhang, X.W. et al. Plant Soil, 310 (2008),pp. 25-40
[39]	Zamir, D., Tadmor, Y. Unequal segregation of nuclear genes in plants Bot. Gaz., 147 (1986),pp. 355-358
[40]	Zhang, X.W., Wu, J., Zhao, J.J. et al. Agr. Sci. Chin., 5 (2006),pp. 265-271