Induction and transmission of wheat-Haynaldia villosa chromosomal translocations

Yaping Cao; Tongde Bie; Xiue Wang; Peidu Chen

doi:10.1016/S1673-8527(08)60120-4

Volume 36 Issue 5

May 2009

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Article Navigation > Journal of Genetics and Genomics > 2009 > 36(5): 313-320

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Induction and transmission of wheat-Haynaldia villosa chromosomal translocations

doi: 10.1016/S1673-8527(08)60120-4

Yaping Cao^{a, b},
Tongde Bie^{a, c},
Xiue Wang^a,
Peidu Chen^a

a
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China
b
Wheat Research Institute, Shanxi Academy of Agricultural Sciences, Linfen 041000, China
c
Yangzhou Academy of Agricultural Sciences, Yangzhou 225007, China

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Corresponding author: E-mail address: pdchen@njau.edu.cn (Peidu Chen)
Received Date: 2008-06-15
Accepted Date: 2009-03-27
Rev Recd Date: 2009-03-26

Available Online: 2009-05-15

Publish Date: 2009-05-20

Abstract

Abstract

In order to develop more wheat-Haynaldia villosa translocations involving different chromosomes and chromosome segments of H. villosa, T. durum-H. villosa amphiploid was irradiated with ⁶⁰Co γ-rays at doses of 800, 1,200, and 1,600 rad. Pollen collected from the spikes 1, 2, and 3 days after irradiation were transferred to emasculated spikes of the common wheat cv. ‘Chinese Spring’. Genomic in situ hybridization was used to identify wheat-H. villosa chromosome translocations in the M₁ generation. Transmission of the identified translocation chromosomes was analyzed in the BC₁, BC₂, and BC₃ generations. The results indicated that all three irradiation doses were highly efficient for inducing wheat-alien translocations without affecting the viability of the M₁ seeds. Within the range of 800–1,600 rad, both the efficiency of translocation induction and the frequency of interstitial chromosome breakage-fusion increased as the irradiation dosage increased. A higher translocation induction frequency was observed using pollen collected from the spikes 1 day after irradiation over that of 2 or 3 days after irradiation. More than 70% of the translocations detected in the M₁ generation were transmitted to the BC₁ through the female gametes. All translocations recovered in the BC₁ generation were recovered in the following BC₂, and BC₃ generations. The transmission ability of different translocation types in different genetic backgrounds showed an order of ‘whole-arm translocation > small alien segment translocation > large alien segment translocation', through either male or female gametes. In general, the transmission ability through the female gametes was higher than that through the male gametes. By this approach, 14 translocation lines that involved different H. villosa chromosomes have been identified in the BC₃ using EST-STS markers, and eight of them were homozygous.
- pollen irradiation,
- chromosomal translocation,
- translocation transmission

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References(34)

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