Analysis of the meiosis in the F1 hybrids of Longiflorum × Asiatic (LA) of lilies (Lilium) using genomic in situ hybridization

Shujun Zhou; Munikote S. Ramanna; Richard G.F. Visser; Jaap M. van Tuyl

doi:10.1016/S1673-8527(08)60091-0

Volume 35 Issue 11

Nov. 2008

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Article Navigation > Journal of Genetics and Genomics > 2008 > 35(11): 687-695

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Analysis of the meiosis in the F1 hybrids of Longiflorum × Asiatic (LA) of lilies (Lilium) using genomic in situ hybridization

doi: 10.1016/S1673-8527(08)60091-0

a
Institute of Landscape, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310029, China
b
Plant Breeding Lab, Wageningen University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands

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Corresponding author: E-mail address: zhou2007@zju.edu.cn (Shujun Zhou)
Received Date: 2008-06-21
Accepted Date: 2008-09-03
Rev Recd Date: 2008-07-14

Available Online: 2008-11-18

Publish Date: 2008-11-20

Abstract

Abstract

Longiflorum and Asiatic lilies of the genus Lilium of the family Liliaceae are two important groups of modern lily cultivars. One of the main trends of lily breeding is to realize introgression between these groups. With cut style pollination and embryo rescue, distant hybrids between the two groups have been obtained. However, the F₁ hybrids are highly sterile or some of them could produce a small number of 2n gametes, and their BC₁ progenies are usually triploids. Dutch lily breeders have selected many cultivars from these BC₁ progenies based on their variation. It is presumably suggested that such variation could be caused by intergenomic recombination and abnormal meiosis during gamete formation in F₁ hybrids of Longiflorum × Asiatic (LA) hybrids in Lilium. Therefore, the meiotic process of ten F₁ LA hybrids was cytologically investigated using genomic in situ hybridization and traditional cytological methods in the present research. The results showed that: at metaphase I, the homoeologous chromosome pairing among different F₁ hybrids ranged from 2.0 to 11.4 bivalents formed by homoeologous chromosomes per pollen mother cell (PMC), and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all bivalents were disjoined and most univalents were divided. Both the disjoined bivalents (half-bivalents) and the divided univalents (sister chromatids) moved to the opposite poles, and then formed two groups of chromosomes; because the two resulting half-bivalents retained their axes in the cell undisturbed, many crossover types, including single crossovers, three strand double crossovers, four strand double crossovers, four strand triple crossovers, and four strand multiple crossovers between the non-sister chromatids in the tetrads of bivalents, were clearly inferred by analyzing the breakpoints on the disjoined bivalents. The present investigation not only explained the reason for sterility of the F₁ LA hybrids and the variation of their BC₁ progenies, but also provided a new method to analyze crossover types in other F₁ interspecific hybrids as well.
- abnormal meiosis,
- crossover,
- 2n gamete

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References(37)

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