Comparison of the Δ12 fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes

Shanlin Yu; Lijuan Pan; Qingli Yang; Ping Min; Zengkai Ren; Hongsheng Zhang

doi:10.1016/S1673-8527(08)60090-9

Volume 35 Issue 11

Nov. 2008

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2008 > 35(11): 679-685

PDF( 739 KB)

Comparison of the Δ12 fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes

doi: 10.1016/S1673-8527(08)60090-9

a
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
b
Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao 266100, China

More Information

Corresponding author: E-mail address: hszhang@njau.edu.cn (Hongsheng Zhang)
Received Date: 2008-07-03
Accepted Date: 2008-08-06
Rev Recd Date: 2008-08-05

Available Online: 2008-11-18

Publish Date: 2008-11-20

Abstract

Abstract

Δ¹² fatty acid desaturase gene has been targeted as a logical candidate controlling the high oleate trait in peanut seeds. By RT-PCR method, the full-length cDNAs of Δ¹² fatty acid desaturase gene were isolated from peanut (Arachis hypogaea L.) genotypes with normal and high ratio of oleic to linoleic acid, which were designated AhFAD2B and AhFAD2B′, respectively. Sequence alignment of their coding regions revealed that an extra A was inserted at the position +442 bp of AhFAD2B′ sequence of high oleic acid genotypes, which resulted in the shift of open reading frame and a truncated protein AhFAD2B′, with the loss of one histidine box involved in metal ion complex required for the reduction of oxygen. Analysis of transcript level showed that the expression of Δ¹² fatty acid desaturase gene in high oleic acid genotype was slightly lower than that in normal genotype. The enzyme activity experiment of yeast (Saccharomyces cerevisiae) cell transformed with AhFAD2B or AhFAD2B′ proved that only AhFAD2B gene product showed significant Δ¹² fatty acid desaturase activity, but AhFAD2B′ gene product did not. These results suggested that the change of AhFAD2B′ gene sequence resulted in lower activity or deactivation of Δ¹² fatty acid desaturase in high oleic acid genotype.
- peanut,
- oleic acid,
- yeast expression,
- function identification

These authors contributed equally to this work.

FullText(HTML)

References(22)

References

[1]	Cahoon, E.B., Mills, L.A., Shanklin, J. J. Bacteriol., 178 (1996),pp. 936-939
[2]	Garcés, R., Mancha, M. Phytochemistry, 30 (1991),pp. 2127-2130
[3]	Gietz, R.D., Schiestl, R.H., Williems, A.R. et al. Studies on the transformation of intact yeast cells by the LiAc/SS- DNA/PEG procedure Yeast, 11 (1995),pp. 355-360
[4]	Hongtrakul, V., Slabaugh, M.B., Knapp, S.J. Crop Sci., 38 (1998),pp. 1245-1249
[5]	Jung, S., Powell, G., Moore, K. et al. Mol. Gen. Genet., 263 (2000),pp. 806-811
[6]	Jung, S., Swift, D., Sengoku, E. et al. Mol. Gen. Genet., 263 (2000),pp. 796-805
[7]	Kabbaj, A., Vervoort, V., Abbott, A.G. et al. Helia, 19 (1996),pp. 1-18
[8]	Kinney, A.J. Development of genetically engineered soybean oils for food applications J. Food Lipids, 3 (1996),pp. 273-292
[9]	Kinney, A.J. Plant as industrial chemical factories-new oils from genetically engineered soybeans Fett/Lipid, 100 (1998),pp. 173-176
[10]	Lee, M.S., Guerra, D.J. Arch. Biochem. Biophys., 315 (1994),pp. 203-211
[11]	Liu, Q., Singh, S.P., Green, A.G. High-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated posttranscriptional gene silencing Plant Physiol., 129 (2002),pp. 1732-1743
[12]	López, Y., Nadaf, H.L., Smith, O.D. et al. Theor. Appl. Genet., 101 (2000),pp. 1131-1138
[13]	Martin, B.A., Rinne, R.W. Plant Physiol., 81 (1986),pp. 41-44
[14]	Martínez-Rivas, J.M., Sperling, P., Lühs, W., and Heinz, E. (1998). Isolation of three different microsomal oleate desaturase cDNA clones from sunflower. Expression studies in normal type and high oleic mutant. In Advances in Plant Lipid Research, J. Sánchez, E. Cerdá-Olmedo, E. Maitínez-Force (eds). Secretariado de Publicaciones de la Universidad de Sevilla, Sevilla, Spain. pp. 137–139.
[15]	Moore, K.M., Knauft, D.A. The inheritance of high-oleic acid in peanut J. Hered., 80 (1989),pp. 252-253
[16]	Norden, A.J., Gorbet, D.W., Knauft, D.A. et al. Variability in oil quality among peanut genotypes in the Florida breeding program Peanut Sci., 14 (1987),pp. 7-11
[17]	Pan, L.J., Yu, S.L., Yang, Q.L. et al. J. Peanut Sci., 36 (2007),pp. 5-10
[18]	Patel, M., Jung, S., Moore, K. et al. Theor. Appl. Genet., 108 (2004),pp. 1492-1502
[19]	Stoujesdijk, P.A., Hurlestone, C., Singh, S.P. et al. Biochem. Soc. Trans., 28 (2000),pp. 938-940
[20]	Töpfer, R., Martini, N., Schell, J. Modification of plant lipid synthesis Science, 268 (1995),pp. 681-686
[21]	Worthington, R.E., Hammons, R.O. J. Am. Oil Chem. Soc., 54 (1977),pp. 105A-108A
[22]	Zhang, H.T., Shan, L., Quan, X.Q. et al. J. Peanut Sci., 35 (2006),pp. 1-7