Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H

Fang Kong; Haiyan Wang; Aizhong Cao; Bi Qin; Jianhui Ji; Suling Wang; Xiu-E Wang

doi:10.1016/S1673-8527(08)60089-2

Volume 35 Issue 11

Nov. 2008

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Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H

doi: 10.1016/S1673-8527(08)60089-2

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China

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Corresponding author: E-mail address: xiuew@njau.edu.cn (Xiu-E Wang)
Received Date: 2008-05-12
Accepted Date: 2008-08-18
Rev Recd Date: 2008-08-01

Available Online: 2008-11-18

Publish Date: 2008-11-20

Abstract

Abstract

In order to transfer useful genes of Hordeum californicum into common wheat (Triticum aestivum L.), the T. aestivum c.v. Chinese Spring (CS)-H. californicum amphiploid was crossed to CS, and its backcrossing and self-fertilized progenies were analyzed by morphological observation, cytological, biochemical and molecular marker techniques. Alien addition lines with two H. californicum chromosomes were identified and their genetic constitution was characterized. STS-PCR analysis using chromosome 2B specific markers indicated that chromosome H3 of H. californicum belongs to homoeologous group 2, and was thus designated 2H. SDS-PAGE showed that chromosome H2 of H. californicum belongs to homoeologous group 5, and was designated 5H. The CS-H. californicum amphiploid and the chromosome addition lines (DA2H and MA5H) identified were evaluated for powdery mildew (Erysiphe graminis f. sp. triticii) resistance in field. The preliminary results indicated that the amphiploid showed higher powdery mildew resistance than CS. However, chromosome addition lines DA2H and MA5H were highly susceptible to powdery mildew, indicating that major powdery mildew resistant genes of H. californicum should be located on chromosomes other than 2H and 5H.
- wheat,
- alien addition lines,
- powdery mildew,
- sequential C-banding/FISH,
- sequence-tag-sites (STS)

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References(23)

References

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