Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a ‘Wangshuibai’ derived population

Amir Mohammad Naji; Mohammad Moghaddam; Mohammad Reza Ghaffari; Hashem Pour Irandoost; Laleh Karimi Farsad; Seyed Mostafa Pirseyedi; Seyed Abolghasem Mohammadi; Behzad Ghareyazie; Mohsen Mardi

doi:10.1016/S1673-8527(08)60083-1

Volume 35 Issue 10

Oct. 2008

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Article Navigation > Journal of Genetics and Genomics > 2008 > 35(10): 625-629

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Validation of EST-derived STS markers localized on Qfhs.ndsu-3BS for Fusarium head blight resistance in wheat using a ‘Wangshuibai’ derived population

doi: 10.1016/S1673-8527(08)60083-1

a
Department of Agronomy and Crop Breeding, Shahed University of Tehran, Tehran 3319118651, Iran
b
Department of Agronomy and Plant Breeding, University of Tabriz, Tabriz 5166616471, Iran
c
Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Karaj 3135933151, Iran

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Corresponding author: E-mail address: mardi@abrii.ac.ir (Mohsen Mardi)
Received Date: 2008-03-11
Accepted Date: 2008-06-23
Rev Recd Date: 2008-06-04

Available Online: 2008-10-18

Publish Date: 2008-10-20

Abstract

Abstract

A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight (FHB) in wheat, have been previously identified in the ‘Sumai 3’/‘Stoa’ population. In this study, we used a ‘Wangshuibai’ (resistant) /‘Seri82’ (susceptible) derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1−Xgwm493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in ‘Wangshuibai’ at 96 h after inoculation was 2.6 times higher than ‘Seri82’. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.
- EST-derived STS markers,
- Fusarium head blight,
- wheat

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References(38)

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