Assignment and expression patterns of porcine muscle-specific isoform of phosphoglycerate mutase gene

Haifang Qiu; Shuhong Zhao; Xuewen Xu; Martine Yerle; Bang Liu

doi:10.1016/S1673-8527(08)60036-3

Volume 35 Issue 5

May 2008

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Article Navigation > Journal of Genetics and Genomics > 2008 > 35(5): 257-260

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Assignment and expression patterns of porcine muscle-specific isoform of phosphoglycerate mutase gene

doi: 10.1016/S1673-8527(08)60036-3

a
Lab of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China
b
INRA, Laboratoire de Génétique Cellulaire, Castanet-Toloson 31326, France

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Corresponding author: E-mail address: liubang@mail.hzau.edu.cn (Bang Liu)
Received Date: 2007-10-30
Accepted Date: 2007-11-13
Rev Recd Date: 2007-11-12

Available Online: 2008-05-20

Publish Date: 2008-05-20

Abstract

Abstract

It has been reported that the muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme; it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In this study, the cDNA of the porcine Pgam2 was cloned. This gene contains an open reading frame of 765 bp encoding a protein of 253 residues, and the predicted protein sequences share high similarity with other mammalians, 96% identity with humans, and 94% identity with mouse and rats. Pgam2 was mapped to SSC18q13-q21 by the RH panel. In this region, there are several QTLs, such as fat ratio, lean percentage, and diameter of muslce fiber, which affect meat production and quality. The reverse transcriptase-polymerase chain reaction revealed that the porcinePgam2 gene was mainly expressed in the muscle tissue (skeletal muscle and cardiac muscle), and was expressed highly at skeletal muscle development stages (embryonic periods: 33, 65, and 90 days post-conception (dpc); postnatal pigs: 4 days and adult). This indicates that the Pgam2 gene plays an important role in muscle growth and development. In addition, it was demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei.
- assignment,
- expression patterns,
- subcellular localization,
- porcine

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References(22)

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