The ethanol response gene Cab45 can modulate the impairment elicited by ethanol and ultraviolet in PC12 cells

Yunfeng Zhu; Quanli Wang; Wangru Xu; Sha Li

doi:10.1016/S1673-8527(08)60021-1

Volume 35 Issue 3

Mar. 2008

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The ethanol response gene Cab45 can modulate the impairment elicited by ethanol and ultraviolet in PC12 cells

doi: 10.1016/S1673-8527(08)60021-1

a
Department of Oncology in Beijing 307 Hospital, Fengtai District, Beijing 100071, China
b
Department of Transfusion in Beijing 307 Hospital, Fengtai District, Beijing 100071, China

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Corresponding author: E-mail address: zhuyf2004@163.com (Yunfeng Zhu)
Received Date: 2007-10-15
Accepted Date: 2007-12-07
Rev Recd Date: 2007-11-27

Available Online: 2008-03-18

Publish Date: 2008-03-20

Abstract

Abstract

High consumption of ethanolic beverages facilitates neurodegeneration, but the mechanism of this process still remained elusive. Suppression subtractive hybridization (SSH) is a technique for detection of rare transcripts. With SSH approach, we identified one ethanol response gene Cab45, which was down-regulated by ethanol with time-dependent manner in B104 cells. The full-length sequence of Cab45 gene was obtained by 5′ -RACE (5′ Rapid Amplification of cDNA Ends) for the first time in rat. Based on the sequence of deduced amino acid of rat Cab45, the alignment was conducted with its counterparts in different species and displayed a high conservation. Using different tissues in rat and cell lines, Cab45 was characterized by a ubiquitous expression and differentiation dependent down-regulation. Given that ethanol facilitates some cell differentiation, we hypothesize that Cab45 is involved in ethanol-mediated differentiation. With transient transfection, the function of Cab45 was investigated by up-regulation and down-regulation in PC12 cells. Ethanol treatment and UV exposure were conducted subsequently and cell proliferations were detected by MTT (Methyl Thiazolyl Tetrazolium) approach. It revealed that the up-regulation of Cab45 modulated the impairment elicited by ethanol and UV in transfected cells. As a member of new calcium binding protein family, the exact role of Cab45 still remains unclear.
- ethanol,
- Cab45,
- calcium-binding protein,
- gene identification,
- subtractive hybridization

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References

[1]	Adickes, E.D., Mollner, T.J., Lockwood, S.K. Closed chamber system for delivery of ethanol to cell cultures Alcohol, 23 (1988),pp. 377-381
[2]	Altman, D.G., Gardner, M.J. Calculating confidence intervals for regression and correlation British Medical Journal, 296 (1988),pp. 1238-1242
[3]	Bates, S., MacCallum, D.M., Bertram, G. et al. J. Biol. Chem., 17 (2005),pp. 23408-23415
[4]	Chen, J.J., Reid, C.E., Band, V. et al. Interaction of papillomavirus E6 oncoproteins with a putative calcium-binding protein Science, 269 (1995),pp. 529-531
[5]	Doll, R., Peto, R. The causes of cancer: quantitative estimates of avoidable risk of cancer in the United States today J. Natl. Cancer Inst., 66 (1981),pp. 1191-1308
[6]	Duguid, J.R., Dinauer, M.C. Library subtraction of in vitro cDNA libraries to identify differentially expressed genes in scrapie infection Nucleic Acids Res., 18 (1990),pp. 2786-2792
[7]	Dutton, G.R., Currie, D.N., Tear, K. An improved method for the bulk isolation of viable perikarya from postnatal cerebellum J. Neurosci. Methods, 3 (1981),pp. 421-427
[8]	Farber, N.B., Olney, J.W. Drugs of abuse that cause developing neurons to commit suicide Brain Res. Dev., 147 (2003),pp. 37-45
[9]	Fein, G., Sclafani, V.D. Cerebral reserve capacity: implications for alcohol and drug abuse Alcohol, 32 (2004),pp. 63-67
[10]	Honore, B., Vorum, H. The CREC family, a novel family of multiple EF-hand, low-affinity Ca(2+)-binding proteins localised to the secretory pathway of mammalian cells FEBS Lett., 466 (2000),pp. 11-18
[11]	Ikonomidou, C., Bittigau, P., Ishimaru, M.J. et al. Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome Science, 287 (2000),pp. 1056-1060
[12]	Jean, Y.G., Christel, M.L., Marc, V. et al. Role of the subcellular localization of ALK tyrosine kinase domain in neuronal differentiation of PC12 cells Journal of Cell Science, 118 (2005),pp. 5811-5823
[13]	Jelliger, K.A. Post mortem studies in Parkinson's disease–is it possible to detect brain areas for specific symptoms? J. Neural Trans. Suppl., 56 (1999),pp. 1-29
[14]	Kim, K.Y., Ke, V., Adkins, L.M. Donepezil for alcohol-related dementia: a case report Pharmacotherapy, 24 (2004),pp. 419-421
[15]	Koivu, T., Laitinen, Riento, K. DNA Seq., 7 (1997),pp. 217-220
[16]	Light, K.E., Brown, D.P., Newton, B.W. et al. Ethanol-induced alterations of neurotrophin receptor expression on Purkinje cells in the neonatal rat cerebellum Brain Res., 924 (2002),pp. 71-81
[17]	Liu, Z., Brattain, M.G., Appert, H. Differential display of reticulocalbin in the highly invasive cell line, MDA-MB-435, versus the poorly invasive cell line, MCF-7 Biochem. Biophys. Res. Commun., 231 (1997),pp. 283-289
[18]	Luchsinger, J.A., Tang, M.X., Siddiqui, M. et al. Alcohol intake and risk of dementia J. Am. Geriatr. Soc., 52 (2004),pp. 540-546
[19]	Luo, J., Miller, M.W. Ethanol inhibits basic fibroblast growth factor-mediated proliferation of C6 astrocytoma cells J. Neuroche., 67 (1996),pp. 1448-1456
[20]	Mufti, S.I. International society for biomedical research on alcoholism: relationship of cell necrosis and proliferation, free radicals and other agents to alcohol-related cancers J. Cancer Res. Clin. Oncol., 119 (1993),pp. 304-305
[21]	Muller, F., Koch, K.W. Calcium-binding proteins and nitric oxide in retinal function and disease Acta Anat. (Basel), 162 (1998),pp. 142-150
[22]	Nixon, R.A., Saito, K.I. Calcium-activated neutral proteinase (calpain) system in aging and Alzheimer's disease Ann. N. Y. Acad. Sci., 747 (1994),pp. 77-91
[23]	Olney, J.W., Wozniak, D.F., Jevtovic-Todorovic, V. et al. Drug-induced apoptotic neurodegeneration in the developing brain Brain Pathol., 12 (2002),pp. 488-498
[24]	Paschen, W., Mengesdorf, T. Endoplasmic reticulum stress response and neurodegeneration Cell Calcium, 38 (2005),pp. 409-415
[25]	Santillano, D.R., Kumar, L.S., Prock, T.L. et al. Ethanol induces cell-cycle activity and reduces stem cell diversity to alter both regenerative capacity and differentiation potential of cerebral cortical neuroepithelial precursors BMC Neurosci., 6 (2005),pp. 59-76
[26]	Scherer, P.E., Lederkremer, G.Z., Williams, S. J. Cell Biol., 133 (1996),pp. 257-268
[27]	Suter, P.M. Alcohol, nutrition and health maintenance selected aspects Proc. Nutr. Soc., 63 (2004),pp. 81-88
[28]	Tønnesen, H., Møller, H., Andersen, J.R. et al. Cancer morbidity in alcohol abusers British J. Cancer, 69 (1994),pp. 327-332
[29]	Uccelletti, D., Farina, F., Pinton, P. et al. Mol. Biol. Cell, 6 (2005),pp. 4636-4647
[30]	Vorum, H., Liu, X., Madsen, P. Biochem. Biophys. Acta, 1386 (1998),pp. 121-131
[31]	Vorum, H., Hager, H., Christensen, M. Human calumenin localizes to the secretory pathway and is secreted to the medium Exp. Cell Res., 248 (1999),pp. 473-481
[32]	Vorum, H., Jacobsen, C., Honoré, B. Calumenin interacts with serum amyloid P component FEBS Lett., 465 (2000),pp. 129-134
[33]	Yabe, D., Nakamura, T., Kanazawa, N. J. Biol. Chem., 272 (1997),pp. 18232-18239
[34]	Young, C., Klocke, B.J., Tenkova, T. et al. Cell Death Diff., 10 (2003),pp. 1148-1155
[35]	Zhu, Y., Lin, H., Li, Z. et al. Modulation of expression of ribosomal protein L7a (rpL7a) by ethanol in human breast cancer cells Breast Cancer Res. Treat., 69 (2001),pp. 29-38