Association of polymorphisms of Nramp1 gene with immune function and production performance of large white pig

Hongmei Wu; Duxue Cheng; Lixian Wang

doi:10.1016/S1673-8527(08)60014-4

Volume 35 Issue 2

Feb. 2008

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Association of polymorphisms of Nramp1 gene with immune function and production performance of large white pig

doi: 10.1016/S1673-8527(08)60014-4

Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China

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Corresponding author: E-mail address: iaswlx@263.net (Lixian Wang)
Received Date: 2007-09-04
Accepted Date: 2007-10-18
Rev Recd Date: 2007-10-08

Available Online: 2008-04-11

Publish Date: 2008-02-20

Abstract

Abstract

The present research was designed to study the association of polymorphism of natural resistance-associated macrophage protein1 (Nramp1) with some immune function and the production performance in Large White pig. The PCR-RFLP technique was applied to analyze the correlation between the polymorphisms of Nramp1 gene and immune function [value of Polymorphonuclear Leukocytes (PMN) obtained by Nitroblue Tetrazolium (NBT) Reduction and effect of Cytotoxin in Monocyte] and production performance in 165 Large White pigs. The results showed that there was oneNde I restriction locus in Large White pig, and both values of PMN by NBT Reduction and effect of Cytotoxin in Monocyte in genotype BB were higher than those in genotype AB (P<0.05). Simultaneously, the weight of 180-day-old pigs with genotype BB was higher than that with genotype AB (P<0.05). The results indicated that there was a significant correlation between different genotypes ofNramp1 gene and Immune function and production performance, and it can be regarded as a candidate gene of disease resistance. All these results provide valuable reference to further studies of pig disease resistance.
- Large White pig,
- immune function,
- production performance

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References(17)

References

[1]	Altschul, S.F., Madden, T.L., Schaffer, A.A. et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res., 25 (1997),pp. 3389-3402
[2]	Belouchi, A., Cellier, M., Kwan, T. et al. Plant Mol. Biol., 29 (1995),pp. 1181-1196
[3]	Blackwell, J.M. Mol. Medicine Today, 2 (1996),pp. 205-211
[4]	Govoni, G., Vidal, S., Cellier, M. et al. Genomics, 27 (1995),pp. 9-19
[5]	Liu, W., Kaiser, M.G., Lamont, S.J. Naturalresistance-associated macrophage protein 1 gene polymorphisms and response to vaccine against or challenge with Salmonella enteritidis in young chicks Poultry Sci., 82 (2003),pp. 259-266
[6]	Marquet, S., Lepage, P., Hudson, T.J. et al. Mammal. Genome, 11 (2000),pp. 755-762
[7]	Paixaõ, T.A., Ferreira, C., Borges, A. et al. Veterinary Immunol. Immunopathol., 109 (2006),pp. 37-42
[8]	Sambrook, J., Russell, D.
[9]	Sanchez, R.E., Altet, L., Sanchez, A. et al. J. Hered., 96 (2005),pp. 755-758
[10]	Shen, G.X., Zhou, R.L.
[11]	Supek, F., Supekova, L., Nelson, H. et al. A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria Proc. Natl. Acad. Sci. USA, 93 (1996),pp. 5105-5110
[12]	Sun, H.S., Wang, L., Rothschild, M.F. et al. Animal Genet., 29 (1998),pp. 138-140
[13]	Tuggle, C.K., Schmitz, C.B., Gingerich-Feil, D. J. Animal Sci., 75 (1997),p. 277
[14]	Vidal, S.M., Pinner, E., Lepage, P. et al. J. Immunol., 157 (1996),pp. 3559-3568
[15]	Wu, Z.F., Luo, W.H., Yang, G.F. et al. DNA Sequence, 18 (2007),pp. 327-333
[16]	Wu, X.W., Liang, Z.H.
[17]	Zhang, G.L., Wu, H., Ross, C.R. et al. Infect Immunity, 68 (2000),pp. 1086-1093