Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization

Yiling Yang; Jiayou Chu; Yupeng Wu; Manli Luo; Xin Xu; Yaling Han; Yan Cai; Qimin Zhan; Mingrong Wang

doi:10.1016/S1673-8527(08)60002-8

Volume 35 Issue 1

Jan. 2008

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Article Navigation > Journal of Genetics and Genomics > 2008 > 35(1): 11-16

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Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization

doi: 10.1016/S1673-8527(08)60002-8

a
Department of Genetics, Institute of Medical Biology, Peking Union Medical College and Chinese Academy of Medical Sciences, Kunming 650118, China
b
State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100021, China

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Corresponding author: E-mail address: chujy@imbcams.com.cn (Jiayou Chu); E-mail address: wangmr04@126.com (Mingrong Wang)
Received Date: 2007-06-15
Accepted Date: 2007-08-15
Rev Recd Date: 2007-08-15

Available Online: 2008-01-25

Publish Date: 2008-01-20

Abstract

Abstract

Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization (M-FISH) for identifying chromosome aberrations in esophageal carcinoma cell line KYSE 410-4, four pools of 6-color whole-chromosome painting probes have been designed and hybridized on the same metaphase spread by four rounds of repetitive FISH. Repetitive 6-color M-FISH was successfully established and the cytogenetic abnormalities in KYSE 410-4 cells were characterized. Chromosome gains occurred at 2q, 3, 8, 17p, and X. An isochromosome 3q was visualized in the cell line, which might be one intermediate mechanism leading to 3p losses and/or 3q gains. Furthermore, 16 structural arrangements were detected, including four derivative chromosomes. The rearrangement of the centromeric regions accounted for approximately 44% of all rearrangements. The results added a more complete and accurate information of the genetic alterations to the classical cytogenetic description of KYSE 410-4 and provided a detailed cytogenetic background data for appropriate use of the cell line. The established 6-color M-FISH was useful for analyzing chromosomes in the whole genome of human tumors.
- KYSE 410-4,
- karyotype,
- esophageal squamous cell carcinoma

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References(23)

References

[1]	Albertson, D.G., Collins, C., McCormick, F. et al. Chromosome aberrations in solid tumors Nat. Genet., 34 (2003),pp. 369-376
[2]	Ando, N., Ozawa, S., Kitagawa, Y. et al. Improvement in the results of surgical treatment of advanced squamous esophageal carcinoma during 15 consecutive years Ann. Surg., 232 (2000),pp. 225-232
[3]	ISCN. (1995). An International System for Human Cytogenetic Nomenclature. 1st edn.
[4]	Jin, Y., Jin, C., Law, S. et al. Cytogenetic and fluorescence in situ hybridization characterization of clonal chromosomal aberrations and CCND1 amplification in esophageal carcinomas Cancer Genet. Cytogenet., 148 (2004),pp. 21-28
[5]	Kuwano, H., Kato, H., Miyazaki, T. et al. Genetic alterations in esophageal cancer Surg. Today, 35 (2005),pp. 7-18
[6]	Lin, M., Smith, L.T., Smiraglia, D.J. et al. DNA copy number gains in head and neck squamous cell carcinoma Oncogene, 25 (2006),pp. 1424-1433
[7]	Muller, S., Neusser, M., Wienberg, J. Chromosome Res., 10 (2002),pp. 223-232
[8]	Nanjundan, M., Nakayama, Y., Cheng, K.W. et al. Amplification of MDS1/EVI1 and EVI1, located in the 3q26.2 amplicon, is associated with favorable patient prognosis in ovarian cancer Cancer Res., 67 (2007),pp. 3074-3084
[9]	Schrock, E., du Manoir, S., Veldman, T. et al. Multicolor spectral karyotyping of human chromosomes Science, 273 (1996),pp. 494-497
[10]	Schulten, H.J., Gunawan, B., Otto, F. et al. Cancer Genet. Cytogenet., 133 (2002),pp. 134-141
[11]	Sebastian, J.L., Rigberg, D.A., Shrivatsan, E. et al. The use of large-scale cDNA analysis to profile differential gene expression in KYSE 410 human esophageal cancer cells after irradiation Bull Exp Biol Med., 130 (2000),pp. 882-885
[12]	Shimada, Y., Imamura, M., Wagata, T. et al. Characterization of 21 newly established esophageal cancer cell lines Cancer, 69 (1992),pp. 277-284
[13]	Smith, L., Liu, S.J., Goodrich, L. et al. Duplication of ATR inhibits MyoD, induces aneuploidy and eliminates radiation-induced G1 arrest Nat. Genet., 19 (1998),pp. 39-46
[14]	Speicher, M.R., Gwyn Ballard, S., Ward, D.C. Karyotyping human chromosomes by combinatorial multi-fluor FISH Nat. Genet., 12 (1996),pp. 368-375
[15]	Stamouli, M.I., Panani, A.D., Ferti, A.D. et al. Cancer Genet. Cytogenet., 149 (2004),pp. 107-113
[16]	Wang, H., Qian, H., Yu, J. et al. Administration of PUMA adenovirus increases the sensitivity of esophageal cancer cells to anticancer drugs Cancer Biol. Ther., 5 (2006),pp. 380-385
[17]	Wang, M.R., Perissel, B., Malet, P. Rehybridization on metaphases studied previously by FISH. An approach to analyze chromosome aberrations Cancer Genet. Cytogenet., 85 (1995),pp. 58-60
[18]	Wei, F., Ni, J., Wu, S.S. et al. Cytogenetic studies of esophageal squamous cell carcinomas in the northern Chinese population by comparative genomic hybridization Cancer Genet. Cytogenet., 138 (2002),pp. 38-43
[19]	Wu, S.S., Liu, J.F., Wang, M.R. Analyzing chromosomal abnormality in primary esophageal cancer by comparative genomic hybridization Ai zheng, 26 (2007),pp. 132-136
[20]	Wu, Y.P., Yang, Y.L., Yang, G.Z. et al. Cancer Genet. Cytogenet., 170 (2006),pp. 102-107
[21]	Yen, C.C., Chen, Y.J., Pan, C.C. et al. Copy number changes of target genes in chromosome 3q25.3-qter of esophageal squamous cell carcinoma: TP63 is amplified in early carcinogenesis but down-regulated as disease progressed World J. Gastroenterol., 11 (2005),pp. 1267-1272
[22]	Yokoi, S., Yasui, K., Iizasa, T. et al. TERC identified as a probable target within the 3q26 amplicon that is detected frequently in non-small cell lung cancers Clin. Cancer Res., 9 (2003),pp. 4705-4713
[23]	Zhen, D.K., Wang, J.Y., Falco, V.M. et al. Poly-FISH: a technique of repeated hybridizations that improves cytogenetic analysis of fetal cells in maternal blood Prenat. Diagn, 18 (1998),pp. 1181-1185