Disrupted ompC Causes Osmosis Sensitivity of Escherichia coli in Alkaline Medium

Yuqi Wang; Lingling Wang; Yirong Sun; Yicai Chen; Lei Zhu; Lixia Guo; Biao Luo; Haihong Wang

doi:10.1016/S1673-8527(07)60129-5

Volume 34 Issue 12

Dec. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(12): 1131-1138

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Disrupted ompC Causes Osmosis Sensitivity of Escherichia coli in Alkaline Medium

doi: 10.1016/S1673-8527(07)60129-5

College of Life Sciences, South China Agricultural University, Guangzhou 510642, China

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Corresponding author: E-mail address: wanghh36@scau.edu.cn (Haihong Wang)
Received Date: 2007-05-17
Rev Recd Date: 2007-06-07

Available Online: 2007-12-31

Publish Date: 2007-12-20

Abstract

Abstract

The Escherichia coli strain DH42 is sensitive to high osmolarity in an alkaline medium. Using mini-Tn5 mutagenesis, construction of mutant strains by homologous recombination and subcloning of DNA fragment techniques, gene ompC was identified as the key factor that, once disrupted, caused osmosis-sensitivity ofE. coli strain DH42 grown in an alkaline medium. Through P1 transduction, a mutant strain, D9 (W3110 ompC:kan), was constructed and growth comparison was performed between DH42 and D9 under different pHs and salt concentrations. The result showed that ompC was necessarily required for hyperosmotic adaptation of E. coli in the alkaline medium.
- osmolarity,
- alkaline,
- pH

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References(22)

References

[1]	Alba, BM, Gross, et al. Mol Microbiol, 3 (2004),pp. 613-619
[2]	Poolman, B, Blount, et al. How do membrane proteins sense water stress? Mol Microbiol, 4 (2002),pp. 889-902
[3]	Wood, JM, Bremer, et al. Osmosensing and osmoregulatory compatible solute accumulation by bacteria Comp Biochem Physiol A Mol Integr Physiol, 3 (2001),pp. 437-460
[4]	Kobayashi, H, Saito, et al. Bacterial strategies to inhabit acidic environments J Gen Appl Microbiol, 5 (2000),pp. 235-243
[5]	Heyde, M, Portalier, et al. Mol Gen Genet, 3 (1987),pp. 511-517
[6]	Thomas, AD, Booth, et al. J Gen Microbiol, 9 (1992),pp. 1829-1835
[7]	Lan, CY, Igo, et al. J Bacteriol, 1 (1998),pp. 171-174
[8]	Liu, X, Ferenci, et al. Microbiology, 11 (2001),pp. 2981-2989
[9]	Pratt, LA, Hsing, et al. Mol Microbiol, 5 (1996),pp. 911-917
[10]	Le Dain, AC, Hase, et al. EMBO J, 14 (1996),pp. 3524-3528
[11]	Nakae, T Outer-membrane permeability of bacteria Crit Rev Microbiol, 1 (1986),pp. 1-62
[12]	Heyde, MP, Laloi, et al. J Bacteriol, 1 (2000),pp. 198-202
[13]	Mizuno, T, Mizushima, et al. Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genes Mol Microbiol, 7 (1990),pp. 1077-1082
[14]	Cai, SJ, Inouye, et al. J Biol Chem, 27 (2002),pp. 24155-24161
[15]	Igo, MM, Slauch, et al. Signal transduction in bacteria: kinases that control gene expression New Biol, 1 (1990),pp. 5-9
[16]	Matsubara, M, Mizuno, et al. Biosci Biotechnol Biochem, 2 (1999),pp. 408-414
[17]	Sato, M, Machida, et al. Appl Environ Microbiol, 3 (2000),pp. 943-947
[18]	Miller, JH
[19]	Castillo-Keller, MP, Vuong, et al. J Bacteriol, 2 (2006),pp. 576-586
[20]	Liu, X, Ferenci, et al. J Bacteriol, 15 (1998),pp. 3917-3922
[21]	Batchelor, E, Goulian, et al. Robustness and the cycle of phosphorylation and dephosphorylation in a two-component regulatory system Proc Natl Acad Sci USA, 2 (2003),pp. 691-696
[22]	Kaeriyama, M, Machida, et al. Lett Appl Microbiol, 3 (2006),pp. 195-201