Population Genetic Diversity in Chinese Pomegranate (Punica granatum L.) Cultivars Revealed by Fluorescent-AFLP Markers

Zhaohe Yuan; Yanlei Yin; Jianlu Qu; Liqin Zhu; Yun Li

doi:10.1016/S1673-8527(07)60121-0

Volume 34 Issue 12

Dec. 2007

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2007 > 34(12): 1061-1071

PDF( 377 KB)

Population Genetic Diversity in Chinese Pomegranate (Punica granatum L.) Cultivars Revealed by Fluorescent-AFLP Markers

doi: 10.1016/S1673-8527(07)60121-0

Shandong Institute of Pomology, Tai'an 271000, China

More Information

Corresponding author: E-mail address: zhyuan88@hotmail.com (Zhaohe Yuan)
Available Online: 2007-12-31
Publish Date: 2007-12-20

Abstract

Abstract

Eighty-five pomegranate (Punica granatum L.) cultivars from six geographical populations located at Shandong, Anhui, Shaanxi, Henan, Yunnan, and Xinjiang Provinces were studied for its population genetic diversity by means of fluorescent-AFLP markers. The results indicated that 135–185 polymorphic loci were amplified by eight pairs of primers at species level. An average of 158.25 polymorphic loci was amplified for each primer combination. The polymorphism percentage ranged from 62.5% to 86.11%, and the average polymorphism percentage was 73.26%. This indicated that there was plentiful genetic diversity in pomegranate cultivars. The genetic diversity at the species level was higher than that at the population level. The order of the genetic diversity was Henan population > Xinjiang population > Shaanxi population > Anhui population > Shandong population > Yunnan population. Variance analysis showed that there was significant difference between populations in genetic diversity. The genetic differentiation coefficient between populations ( G_ST) was 0.2018, which indicated that gene differentiation was mainly within the population, and between populations, it accounted for 20.18% of the total variation. Gene flow (Nm) between the populations measured was 1.9027 based on the genetic differentiation coefficient between populations, indicating that there was mild gene flow between populations. The UPGMA cluster analysis showed that most accessions from the same population were clustered together, but there was partly gene exchange. All genetic parameters indicated that there was plentiful genetic diversity in pomegranate cultivars in China, of which Henan population was significantly higher than the other populations, and it had wide application foreground in pomegranate breeding in China.
- pomegranate,
- AFLP marker,
- genetic diversity,
- population genetic structure

FullText(HTML)

References(24)

References

[1]	Gil, MI, Tomás-Barberán, et al. Antioxidant acivity of pomegranate juice and its relationship with phenolic composition and processing J Agric Food Chem, 48 (2000),pp. 4581-4589
[2]	Ben Nasr, N, Ayed, et al. Quantitative determination of the polyphenolic content of pomegranate peel Z Lebensm Unters Forsch, 203 (1996),pp. 374-378
[3]	Zhang, J, Zhan, et al. Chung Kuo Chung Yao Tsa Chih, 20 (1995),pp. 556-558
[4]	Gong, XM, Zhang, et al. Economic characteristics of fruits in pomegranate cultivars in China Journal of Plant Genetic Resources, 5 (2004),pp. 17-21
[5]	Zhou, GJ, Yuan, et al. Status and prospect of pomegranate production in China Southwest China Journal of Agricultural Sciences, 8 (1995),pp. 111-116
[6]	Feng, YZ, Chen, et al.
[7]	Zabeau M, Vos P. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application, no. 92402629.7. Publ no. 0534858 A1. 1993.
[8]	Waugh, R, Bonar, et al. Homology of AFLP products in three mapping populations of barley Mol Gen Genet, 255 (1997),pp. 311-321
[9]	Schut, JW, Qi, et al. Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley Theor Appl Genet, 95 (1997),pp. 1161-1168
[10]	Yuan, LX, Fu, et al. Comparison of genetic diversity among maize inbred lines based on RFLPs, SSRs, AFLPs and RAPDs Acta Genet Sin, 27 (2000),pp. 725-733
[11]	Doyle, JJ, Doyle, et al. A rapid DNA isolation procedure for small quantities of fresh leaf tissue Phytochem Bull, 19 (1987),pp. 11-15
[12]	Francis, CY, Yang, et al.
[13]	Nei, M
[14]	Sun, CQ, Wang, et al. Scientia Agricultura Sinica, 30 (1997),pp. 37-44
[15]	Nei, M Analysis of gene diversity in subdivided populations Proc Natl Acad Sci USA, 70 (1973),pp. 3321-3323
[16]	Xu, DH, Wahyuni, et al. Genet Resour Crop Evol, 53 (2006),pp. 883-889
[17]	Wang, L, Xing, et al. Acta Genet Sin, 33 (2006),pp. 1020-1026
[18]	Liu, Y, Sun, et al. Assessment of the genetic diversity of pummelo germplasms using AFLP and SSR markers Scientia Agricultura Sinica, 38 (2005),pp. 2308-2315
[19]	He, TM, Chen, et al. Genet Resour Crop Evol, 54 (2007),pp. 563-572
[20]	Cheng, ZP, Huang, et al. Journal of Wuhan Botanical Research, 22 (2004),pp. 27-32
[21]	Grant, V
[22]	Hamrick, JL
[23]	Feng, YZ, Song, et al. Pomegranate in China: the status of production and research and suggestions for industry development Deciduous Fruits (2006),pp. 11-15
[24]	Qiu, YX, Fu, et al. China Journal of Chinese Materia Medica, 28 (2003),pp. 598-603