Effect of Menopause on Gene Expression Profiles of Circulating Monocytes: A Pilot in vivo Microarray Study

Volodymyr Dvornyk; Yaozhong Liu; Yan Lu; Hui Shen; Joan M Lappe; Robert R Recker; Hongwen Deng; Shufeng Lei

doi:10.1016/S1673-8527(07)60110-6

Volume 34 Issue 11

Nov. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(11): 974-983

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Effect of Menopause on Gene Expression Profiles of Circulating Monocytes: A Pilot in vivo Microarray Study

doi: 10.1016/S1673-8527(07)60110-6

a
Laboratory of Molecular and Statistical Genetics, College of Life Sciences, Hunan Normal University, Changsha 410081, China
b
Department of Biological Sciences, Kent State University, Kent, OH 44242, USA
c
Osteoporosis Research Center, Creighton University Medical Center, Omaha, NE 68131, USA
d
Departments of Basic Medical Science, School of Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USA

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Corresponding author: E-mail address: hwdeng@hunnu.edu.cn (Hongwen Deng)
Received Date: 2007-02-28
Accepted Date: 2007-08-09

Available Online: 2007-11-22

Publish Date: 2007-11-20

Abstract

Abstract

Menopause is one of the key physiological events in the female life and can increase the risk for a number of complex autoimmune, neurodegenerative, metabolic, and cardiovascular disorders. Circulating monocytes can differentiate into various cell types and play an important role in tissue morphogenesis and immune response. We studied gene expression profiles of peripheral blood monocytes in healthy pre- and postmenopausal women using Affymetrix Human U133A GeneChip array that contains probes for ∼14,500 genes. Comparative analyses between the samples showed that 20 genes were up- and 20 were down-regulated. Of these genes, 28 were classified into six major GO categories relevant to such biological processes as the cell proliferation, immune response, cellular metabolism, and the others. The remaining 12 genes have yet unidentified biological functions. Our results support the hypothesis that functional state of circulating monocytes is indeed affected by menopause, and resulting changes may be determined through the genomewide gene expression profiling. Several differentially expressed genes identified in this study may be candidates for further studies of menopause-associated systemic autoimmune, neurodegenerative, and cardiovascular disorders. Our study is only the first attempt in this direction, but it lays a basis for further research.
- menopause,
- monocytes,
- microarrays,
- differential gene expression

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References(59)

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