Expression Patterns of the Cell Junction-associated Genes During Rat Liver Regeneration

Honglei Li; Xiaoguang Chen; Fuchun Zhang; Ji Ma; Cunshuan Xu

doi:10.1016/S1673-8527(07)60101-5

Volume 34 Issue 10

Oct. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(10): 892-908

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Expression Patterns of the Cell Junction-associated Genes During Rat Liver Regeneration

doi: 10.1016/S1673-8527(07)60101-5

a
College of Life Science and Technology, Xinjiang University, Urmuqi 830046, China
b
Co-constructing Key Laboratory for Cell Differentiation Regulation by Province and the MOST, Xinxiang 453007, China
c
College of Life Science, Henan Normal University, Xinxiang 453007, China

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Corresponding author: E-mail address: xucs@x263.net (Cunshuan Xu)
Received Date: 2007-02-06
Accepted Date: 2007-04-24

Available Online: 2007-10-16

Publish Date: 2007-10-20

Abstract

Abstract

To study actions of the genes associated with tight junction, adherent junction, focal adhesion, and gap junction during liver regeneration (LR), these genes were obtained by collecting data from databases and thesis, and their expression profiles in rat regenerating liver were detected employing Rat Genome 230 2.0 array. Next the LR-associated genes were identified by comparing the difference between sham operation (SO) and partial hepatectomy (PH) groups. 79, 53, 109, 53 genes involved in the above four junctions were found to be LR-associated. The initial and total expression numbers of these genes occurring in the initial phase of LR, G0/G1, cell proliferation, cell differentiation, and structure-functional rebuilding were 124, 43, 122, 10, and 249, 145, 957, 306, respectively, illustrating that genes were initially expressed mainly in the initiation stage, and functioned in different phases. Up-regulation and down-regulation to a total of 972 and 540 times, as well as, 41 types of expression patterns showed that the physiological and biochemical activities were diverse and complicated in LR. According to the data, there was an increase in the forepart and prophase, but a decrease in late-metaphase and anaphase for gap junction assembly. Focal adhesion formation displayed an enhancement in forepart, prophase, and anaphase; and formation of tight junctions and adherent junctions last throughout the LR.
- partial hepatectomy (PH),
- rat genome 230 2.0 array,
- cell junction,
- genes associated with LR

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References(50)

References

[1]	Taub, R Liver regeneration: from myth to mechanism Nat Rev Mol Cell Biol, 5 (2004),pp. 836-847
[2]	Higgins, GM, Anderson, et al. Experimental pathology of the liver: Restoration of the liver of the white rat following partial surgical removal Arch Pathol, 12 (1931),pp. 186-202
[3]	Fausto, N, Campbell, et al. Liver regeneration Hepatology, 43 (2006),pp. S45-S53
[4]	Michalopoulos, GK, DeFrances, et al. Liver regeneration Adv Biochem Eng Biotechnol, 93 (2005),pp. 101-134
[5]	Fausto, N, Campbell, et al. The role of hepatocytes and oval cells in liver regeneration and repopulation Mech Dev, 120 (2003),pp. 117-130
[6]	Itoh, M, Bissell, et al. The organization of tight junctions in epithelia: implications for mammary gland biology and breast tumorigenesis J Mammary Gland Biol Neoplasia, 8 (2003),pp. 449-462
[7]	Beaudoin, GM Con-nectin axons and dendrites J Cell Biol, 174 (2006),pp. 7-9
[8]	Nusrat, A, Parkos, et al. Tight junctions are membrane microdomains J Cell Sci, 113 (2000),pp. 1771-1781
[9]	Gao, L, Joberty, et al. Assembly of epithelial tight junctions is negatively regulated by Par6 Curr Biol, 12 (2002),pp. 221-225
[10]	Tsukita, S, Furuse, et al. Overcoming barriers in the study of tight junction functions: from occludin to claudin Genes Cells, 3 (1998),pp. 569-573
[11]	Balkovetz, DF Claudins at the gate: determinants of renal epithelial tight junction paracellular permeability Am J Physiol Renal Physiol, 290 (2006),pp. F572-F579
[12]	, Faralli, JA, et al. Effect of heparin II domain of fibronectin on actin cytoskeleton and adherens junctions in human trabecular meshwork cells Invest Ophthalmol Vis Sci, 47 (2006),pp. 2924-2931
[13]	Miyake, Y, Inoue, et al. Actomyosin tension is required for correct recruitment of adherens junction components and zonula occludens formation Exp Cell Res, 312 (2006),pp. 1637-1650
[14]	Liwosz, A, Lei, et al. N-glycosylation affects the molecular organization and stability of E-cadherin junctions J Biol Chem, 281 (2006),pp. 23138-23149
[15]	Pilot, F, Philippe, et al. Spatial control of actin organization at adherens junctions by a synaptotagmin-like protein Btsz Nature, 442 (2006),pp. 580-584
[16]	Kiener, HP, Stipp, et al. The cadherin-11 cytoplasmic juxtamembrane domain promotes alpha-catenin turnover at adherens junctions and intercellular motility Mol Biol Cell, 17 (2006),pp. 2366-2376
[17]	Chen, CS, Tan, et al. Mechanotransduction at cell-matrix and cell-cell contacts Annu Rev Biomed Eng, 6 (2004),pp. 275-302
[18]	Blattner, SM, Kretzler, et al. Integrin-linked kinase in renal disease: connecting cell-matrix interaction to the cytoskeleton Curr Opin Nephrol Hypertens, 14 (2005),pp. 404-410
[19]	Grimston, SK, Screen, et al. Role of connexin43 in osteoblast response to physical load Ann N Y Acad Sci, 1068 (2006),pp. 214-224
[20]	Liu, F, Arce, et al. Nanomechanics of hemichannel conformations: connexin flexibility underlying channel opening and closing J Biol Chem, 281 (2006),pp. 23207-23217
[21]	Shimizu, K, Shimoichi, et al. Reduced expression of the Connexin26 gene and its aberrant DNA methylation in rat lung adenocarcinomas induced by N-nitrosobis (2-Hydroxypropyl)amine Mol Carcinog, 45 (2006),pp. 710-714
[22]	Xu, CS, Chang, et al. Expressed genes in regenerating rat liver after partial hepatectomy World J Gastroenterol, 11 (2005),pp. 2932-2940
[23]	Dransfeld, O, Gehrmann, et al. Oligonucleotide microarray analysis of differential transporter regulation in the regenerating rat liver Liver Int, 25 (2005),pp. 1243-1258
[24]	Collins, JF Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum Biol Res, 39 (2006),pp. 25-37
[25]	Yue, H, Eastman, et al. An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression Nucleic Acids Res, 29 (2001),p. E41
[26]	Knepp, JH, Geahr, et al. Comparison of automated and manual nucleic acid extraction methods for detection of enterovirus RNA J Clin Microbiol, 41 (2003),pp. 3532-3536
[27]	Nuyts, S, Van Mellaert, et al. Efficient isolation of total RNA from Clostridium without DNA contamination J Microbiol Methods, 44 (2001),pp. 235-238
[28]	Arkin, A, Ross, et al. Genetics, 149 (1998),pp. 1633-1648
[29]	Li, L, Roden, et al. Reproducibility, fidelity, and discriminant validity of mRNA amplification for microarray analysis from primary hematopoietic cells J Mol Diagn, 7 (2005),pp. 48-56
[30]	Eisen, MB, Spellman, et al. Cluster analysis and display of genome-wide expression patterns Proc Natl Acad Sci USA, 95 (1998),pp. 14863-14868
[31]	Werner, T Cluster analysis and promoter modelling as bioinformatics tools for the identification of target genes from expression array data Pharmacogenomics, 2 (2001),pp. 25-36
[32]	Tarulli, GA, Stanton, et al. Adult sertoli cells are not terminally differentiated in the Djungarian hamster: effect of FSH on proliferation and junction protein organization Biol Reprod, 74 (2006),pp. 798-806
[33]	Carninci, P, Kasukawa, et al. The transcriptional landscape of the mammalian genome Science, 309 (2005),pp. 1559-1563
[34]	Makarova, O, Roh, et al. Mammalian Crumbs3 is a small transmembrane protein linked to protein associated with Lin-7 (Pals1) Gene, 302 (2003),pp. 21-29
[35]	Hewitt, KJ, Agarwal, et al. The claudin gene family: expression in normal and neoplastic tissues BMC Cancer, 6 (2006),p. 186
[36]	Herouy, Y, Kahle, et al. Tight junctions and compression therapy in chronic venous insufficiency Int J Mol Med, 18 (2006),pp. 215-219
[37]	Peignon, G, Thenet, et al. E-cadherin-dependent transcriptional control of apolipoprotein A-IV gene expression in intestinal epithelial cells: a role for the hepatic nuclear factor 4 J Biol Chem, 281 (2006),pp. 3560-3568
[38]	Zhang, ZG, Zhang, et al. Correlation of VEGF and angiopoietin expression with disruption of blood-brain barrier and angiogenesis after focal cerebral ischemia J Cereb Blood Flow Metab, 22 (2002),pp. 379-392
[39]	Juriloff, DM, Harris, et al. Mouse models for neural tube closure defects Hum Mol Genet, 9 (2000),pp. 993-1000
[40]	Xu, CS, Zhang, et al. The structure and function of ADAMs Progress in Natural Science, 11 (2001),pp. 902-908
[41]	Tanaka, H, Katoh, et al. Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity J Biol Chem, 281 (2006),pp. 10355-10364
[42]	Bamji, SX Cadherins: actin with the cytoskeleton to form synapses Neuron, 47 (2005),pp. 175-178
[43]	Kausalya, PJ, Phua, et al. Association of ARVCF with zonula occludens (ZO)-1 and ZO-2: binding to PDZ-domain proteins and cell-cell adhesion regulate plasma membrane and nuclear localization of ARVCF Mol Biol Cell, 15 (2004),pp. 5503-5515
[44]	Heck, N, Klausmeyer, et al. Cortical neurons express PSI, a novel isoform of phosphacan/RPTPbeta Cell Tissue Res, 321 (2005),pp. 323-333
[45]	Kazanietz, MG Eyes wide shut: protein kinase C isozymes are not the only receptors for the phorbol ester tumor promoters Mol Carcinog, 28 (2000),pp. 5-11
[46]	Cruciani, V, Mikalsen, et al. The vertebrate connexin family Cell Mol Life Sci, 63 (2006),pp. 1125-1140
[47]	Lee, MS, Kim, et al. Integrin signaling and cell spreading mediated by phorbol 12-myristate 13-acetate treatment J Cell Biochem, 99 (2006),pp. 88-95
[48]	Hunt, PN, Gust, et al. Primary role of the serotonergic midline system in synchronized spontaneous activity during development of the embryonic mouse hindbrain J Neurobiol, 66 (2006),pp. 1239-1252
[49]	Visel, A, Alvarez-Bolado, et al. Comprehensive analysis of the expression patterns of the adenylate cyclase gene family in the developing and adult mouse brain J Comp Neurol, 496 (2006),pp. 684-697
[50]	Barros, JC, Marshall, et al. Activation of either ERK1/2 or ERK5 MAP kinase pathways can lead to disruption of the actin cytoskeleton J Cell Sci, 118 (2005),pp. 1663-1671