Genetic Diversity of Maize (Zea mays L.) Landraces from Southwest China Based on SSR Data

Qilun Yao; Kecheng Yang; Guangtang Pan; Tingzhao Rong

doi:10.1016/S1673-8527(07)60096-4

Volume 34 Issue 9

Sep. 2007

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Genetic Diversity of Maize (Zea mays L.) Landraces from Southwest China Based on SSR Data

doi: 10.1016/S1673-8527(07)60096-4

a
Maize Research Institute, Sichuan Agricultural University, Ya'an 625014, China
b
Department of Life Science, Yangtze Normal College, Fuling 408003, China

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Corresponding author: E-mail address: yangkc2006@yahoo.com.cn (Kecheng Yang)
Received Date: 2006-11-22
Accepted Date: 2006-12-28

Available Online: 2007-09-18

Publish Date: 2007-09-20

Abstract

Abstract

Genetic diversity of 54 maize landraces from southwest China was tested using bulk DNA samples and 42 microsatellite (SSR) loci distributed on 10 chromosomes of maize. A total of 256 alleles were detected among the landraces. At each locus, the number of alleles varied from 2 to 9, with an average of 6.1. On the basis of the genetic similarity coefficients, clustering analysis separated the landraces into four groups. The landraces collected from the same region were mostly grouped together. To reveal the genetic structure and genetic diversity within landraces, 165 individuals from 11 landraces were analyzed. Individual DNA samples proved to be superior to bulk DNA samples in identifying genetic diversity of landraces. A total of 330 alleles were detected in the 11 landraces. According to the results of the individual DNA sampling analysis, estimates of the mean number of alleles ‘A’, the effective allelic number ‘’, the observed heterozygosity ‘’, and expected heterozygosity ‘’ were 7.86, 3.90, 0.69, and 0.37, respectively. An obvious genetic deviation from Hardy-Weinberg expectation was observed both among and within landraces and a considerable genetic variation was revealed within rather than among landraces. In addition, genetic diversity of landraces was greater in Sichuan than in the other three regions. It can be concluded that maize landraces in southwest China were initially introduced to Sichuan and from there to adjacent areas.
- genetic diversity,
- maize landraces,
- SSR

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References(22)

References

[1]	SK, William, RC, et al.
[2]	LL, Darrah, MS, et al. The United States farm corn germplasm base and commercial breeding strategies Crop Sci, 26 (1986),pp. 1109-1113
[3]	Y, Li, J, et al. Genetic diversity and relationships among Chinese maize inbred lines revealed by SSR markers Maydica, 47 (2002),pp. 93-101
[4]	SJ, Liu, TZ, et al. Acta Agronomica Sinica, 30 (2004),pp. 221-226
[5]	TZ, Yong, WS, et al.
[6]	FC, Reif, AE, et al. Genetic distance based on simple sequence repeats and heterosis in tropical maize populations Crop Sci, 43 (2003),pp. 1275-1282
[7]	JSC, Smith, ECL, et al. Theo Appl Genet, 95 (1997),pp. 163-173
[8]	EM, Sharon, S, et al. Application of multiplex PCR and fluorescence-based, semi-automated allele sizing technology for genotyping plant genetic resources Crop Sci, 37 (1997),pp. 617-624
[9]	M, Heckenberger, AE, et al. Variation of DNA fingerprints among accessions within maize inbred lines with regard to the identification of essentially derived varieties. I. Genetic and technical sources of variation in SSR data Mol Breed, 10 (2002),pp. 181-191
[10]	OR, Scott, AJ, et al. Extraction of DNA from plant tissue Plant Mol Bio Manual, A6 (1988),pp. 1-6
[11]	M, Nei Analysis of gene diversity in subdivided populations Proc Natl Acad Sci USA, 70 (1973),pp. 3321-3323
[12]	S, Wright Isolation by distance Genetics, 28 (1943),pp. 114-138
[13]	L, Tao, J, et al.
[14]	YJ, Liu, YB, et al. Comparative analysis of genetic diversity in landraces of waxy maize from Yunnan and Guizhou using SSR markers Sci Agric Sin, 4 (2005),pp. 648-653
[15]	YS, Wu, YL, et al. Genetic diversity of waxy corn and popcorn landraces in Yunnan by SSR markers Acta Agron Sin, 30 (2004),pp. 36-42
[16]	S, Dreisigacker, P, et al. Genetic diversity among and within CIMMYT wheat landrace accessions investigated with SSRs and implications for plant genetic resources management Crop Sci, 45 (2005),pp. 653-661
[17]	SK, Wu, MH, et al.
[18]	RW, Michelmore, I, et al. Identification of markers linked to disease resistance genes by bulk segregant analysis: a rapid method to detect markers in specific genomic regions using segregating populations Proc Natl Acad Sci USA, 88 (1991),pp. 9828-9832
[19]	Y, Loarce, R, et al. A comparative analysis of genetic relationships between rye cultivars using RFLP and RAPD markers Euphytica, 88 (1996),pp. 107-115
[20]	P, Dubreuil, C, et al. Evaluation of a DNA pool-sampling strategy for estimating the RFLP diversity of maize populations Plant Mol Biol Rep, 17 (1999),pp. 123-138
[21]	C, Rebourg, P, et al. Genetic diversity among maize populations: bulk RFLP analysis of 65 accessions Maydica, 44 (1999),pp. 237-249
[22]	X, Liu, MS, et al. Sampling method for genetic variation survey in maize populations detected by SSR markers Acta Agron Sin, 31 (2005),pp. 858-863