C-terminal 76 Amino Acids of eRF3 Are Not Required for the Binding of Release Factor eRF1a from Euplotes octocarinatus

Li Song; Yuyao Wang; Baofeng Chai; Wei Wang; Aihua Liang

doi:10.1016/S1673-8527(07)60053-8

Volume 34 Issue 6

Jun. 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(6): 486-490

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C-terminal 76 Amino Acids of eRF3 Are Not Required for the Binding of Release Factor eRF1a from Euplotes octocarinatus

doi: 10.1016/S1673-8527(07)60053-8

Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China

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Corresponding author: E-mail address: aliang@sxu.edu.cn (Aihua Liang)
Received Date: 2006-09-25
Accepted Date: 2006-12-11

Available Online: 2007-06-27

Publish Date: 2007-06-20

Abstract

Abstract

Termination of translation in eukaryotes requires two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, whereas eRF3 is a ribosome-dependent and eRF1-dependent GTPase. Polypeptide release factor eRF3 consists of N-terminal variable region and C-terminal conserved part. C-terminal part of eRF3 is responsible for termination of the translation. In the present study, the C-terminal of Euplotes octocarinatus eRF3 (eRF3C) and truncate eRF3C lacking 76 amino acids in C-terminal (eRF3Ct) were expressed in Escherichia coli. The recombinant GST-eRF3C and GST-eRF3Ct polypeptides were purified by affinity chromatography using glutathione Sepharose 4B column. After enzymatic cleavage of GST tail, the eRF3C and eRF3Ct protein were obtained. Pull-down analysis showed that the recombinant GST-eRF3C and GST-eRF3Ct polypeptides interacted with E. octocarinatus polypeptide chain release factor eRF1a. This result suggested that the C-terminal of eRF3 having 76 amino acids were not required for the binding of eRF1a in Euplotes octocarinatus.
- polypeptide release factor 3,
- expression,
- pull-down assay

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References(17)

References

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