Genetic Polymorphism of TLR4 Gene and Correlation with Mastitis in Cattle

Xingping Wang; Shangzhong Xu; Xue Gao; Hongyan Ren; Jinbao Chen

doi:10.1016/S1673-8527(07)60044-7

Volume 34 Issue 5

May 2007

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Article Navigation > Journal of Genetics and Genomics > 2007 > 34(5): 406-412

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Genetic Polymorphism of TLR4 Gene and Correlation with Mastitis in Cattle

doi: 10.1016/S1673-8527(07)60044-7

a
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China
b
Animal Science-Technology College of Northwest A & F University, Yangling 712100, China

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Corresponding author: E-mail address: simmenta@vip.sina.com (Shangzhong Xu)
Received Date: 2006-06-02
Accepted Date: 2006-07-18

Available Online: 2007-06-07

Publish Date: 2007-05-20

Abstract

Abstract

Toll-like receptor 4 (TLR4) recognizes pathogen ligands and mediates signaling to initiate innate and adaptive immune responses. In this experiment, a 316 bp and 382 bp fragments of TLR4 gene named T4CRBR1 and T4CRBR2, of Chinese Holstein, Sanhe cattle, and Chinese Simmental was amplified by polymerase chain reaction (PCR), respectively. The genetic polymorphisms in the three populations were detected by Single-Strand Conformational Polymorphism (SSCP) in the first locus and by digesting the fragments with restriction endonuclease Alu? in the second one. Results showed that both alleles (A and B) of two loci were found in all the three populations and the value of polymorphism information content (PIC) indicated that these were a moderate polymorphism. Statistical results of χ² test indicated that two polymorphism sites in the three populations fitted with Hardy–Weinberg equilibrium (P > 0.05). After sequencing, A-G single nucleotide polymorphism (SNP) was identified at nucleotide 4,525 in intron 1 of TLR4 gene and C-T SNP was identified at nucleotide 1,397 in exon 3 of TLR4 gene. Meanwhile, the effect of polymorphism of TLR4 gene on somatic cell score (SCS) was analyzed, the results indicated that the cattle with allele A in T4CRBR1 showed lower somatic cell score than that of allele B (P < 0.05). In short, the allele A might play an important role in mastitis resistance in bovine.
- bovine,
- SSCP,
- RFLP,
- mastitis,
- somatic cell count,
- somatic cell score

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References(16)

References

[1]	Ruegg, PL Investigation of mastitis problems on farms Vet Clin North Am: Food Anim Pract, 19 (2003),pp. 47-73
[2]	Gao, SX, Xu, et al. Acta Veterinaria et Zootechnica Sinica, 37 (2006),pp. 317-320
[3]	Cao, SZ, Li, et al. Construction of mastitis resistant cDNA library in dairy cows using suppression subtractive hybridization Acta Veterinaria et Zootechnica Sinica, 36 (2005),pp. 526-530
[4]	Shizuo, A, Kiyoshi, et al. Toll-like receptors: critical proteins linking innate and acquired immunity Nature Immunology, 2 (2001),pp. 675-680
[5]	White, AN, Kata, et al. Comparative fine maps of bovine toll-like receptor 4 and toll-like receptor 2 regions Mammalian Genome, 14 (2003),pp. 149-155
[6]	McGuire, K, Jones, et al. Radiation hybrid mapping of all 10 characterized bovine Toll-like receptors Animal Genetics, 37 (2005),pp. 47-50
[7]	Goldammer, T, Zerbe, et al. Mastitis increases mammary mRNA abundance of β-defensin 5, Toll-like receptor 2 (TLR2), and TLR4 but not TLR9 in cattle Clinical and Diagnostic Laboratory Immunology, 11 (2004),pp. 174-185
[8]	White, SN, Taylor, et al. Haplotype variation in bovine Toll-like receptor 4 and computational prediction of a positively selected ligand-binding domain Proc Natl Acad Sci USA, 100 (2003),pp. 10364-10369
[9]	Shook GE. Approaches to summarizing somatic cell counts which improve interpretability. Proc Natl Mastitis Countil, Arlington, VA, 1982, 150–166.
[10]	Wiggans, GR, Shook, et al. A lactation measure of somatic cell count J dairy Sci, 70 (1987),pp. 2666-2672
[11]	Beuder, B Toll-like receptors: how they work and what they do Curr Opin Hematol, 9 (2002),pp. 2-10
[12]	Li, Y, Ning, et al. Toll like receptors and immunity Fore Med Sci Select Immunol, 27 (2004),pp. 228-230
[13]	Barkema, HW, Van Der Ploeg, et al. Management style and its association with bulk milk somatic cell count and incidence rate of clinical mastitis J dairy Sci, 82 (1999),pp. 1655-1663
[14]	Shook, GE, Schutz, et al. Selection on somatic cell score to improve resistance to mastitis in the United States J Dairy Sci, 77 (1994),pp. 648-658
[15]	Banos, G, Shook, et al. Genotype by environment interaction and genetic correlations among parities for somatic cell count and milk yield J Dairy Sci, 73 (1990),pp. 2563-2573
[16]	Shook, GE Selection for disease resistance J Dairy Sci, 72 (1989),pp. 1349-1362