Characterization of the Full-length cDNA, Chromosomal Localization, and Polymorphism of the Porcine RPLP0 Gene

Xiao Wu; Shulin Yang; Zhengmao Zhu; Shutang Feng; Kui Li

doi:10.1016/S1673-8527(07)60011-3

Volume 34 Issue 2

Feb. 2007

Turn off MathJax

Article Contents

Article Navigation > Journal of Genetics and Genomics > 2007 > 34(2): 104-108

PDF( 291 KB)

Characterization of the Full-length cDNA, Chromosomal Localization, and Polymorphism of the Porcine RPLP0 Gene

doi: 10.1016/S1673-8527(07)60011-3

a
Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
b
Department of Animal Genetics and Breeding, School of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

More Information

Corresponding author: E-mail address: kuili@iascaas.net.cn (Kui Li)
Received Date: 2006-02-10
Accepted Date: 2006-07-30

Available Online: 2007-04-18

Publish Date: 2007-02-20

Abstract

Abstract

RPLP0 gene encodes the acidic ribosomal phosphoprotein large P0 subunit, which is a component of the 60S subunit. The full-length cDNA sequence of porcine RPLP0 was obtained from skeletal muscle of fetal pig cDNA library and deposited in GenBank. The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homologues. A C/A single nucleotide substitution in exon 5 was detected as Csp6?polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency diversity among Tongcheng, Xiaomeishan, Yushan, Large White, Landrace, and Duroc breeds. Analyses of somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel showed that the RPLP0 gene was mapped to SSC 14q22-q24 and was closely linked to locus SW1321 (25 cR, LOD = 14.54).
- RPLP0,
- porcine,
- IMpRH,
- SCHP,
- polymorphisms

FullText(HTML)

References(14)

References

[1]	Wool, IG, Chan, et al. Structure and evolution of mammalian ribosomal proteins Biochem Cell Biol, 73 (1995),pp. 933-947
[2]	Kuhlberger, R, Piepersberg, et al. Alteration of ribosomal protein L6 in gentamicin-resistant strains of Escherichia coli: effects on fidelity of protein synthesis Biochemistry, 18 (1979),pp. 187-193
[3]	Zeng, JZ, Hong, et al. Cloning and expression of ribosomal protein L26 in hepatocellular carcinoma Chin J Exp Surg, 18 (2001),pp. 108-109
[4]	Wang, JS, Yang, et al. Analysis of nuclear localization signal (NLS) in ribosomal protein L6/Taxreb107 Prog Biochem Biophys, 29 (2002),pp. 144-148
[5]	Zhang, ZL, Harrison, et al. Identification and analysis of over 2000 ribosomal protein pseudogenes in the human genome GenomeResearch, 12 (2002),pp. 1466-1482
[6]	Kenmochi, N, Kawaguchi, et al. A map of 75 human Ribosomal protein genes Genome Research, 5 (1998),pp. 509-523
[7]	Irobi, J, Nelis, et al. Exclusion of 5 functional candidate genes for distal hereditary motor neuropathy type II (distal HMN II) linked to 12q24.3 Ann Hum Genet, 65 (2001),pp. 517-529
[8]	Li, JH, Cui, et al. Single nucleotide polymorphism analysis in sow mu opioid receptor gene exon ? Acta Genetica Sinica, 30 (2003),pp. 30-34
[9]	Zhou, B, Wang, et al. Advances in allelic polymorphism at class ? of the pig MHC(SLA) Hereditas, 25 (2003),pp. 611-614
[10]	Zhu, ZM, Zhang, et al. Animal Genetics, 36 (2005),pp. 191-195
[11]	Chevalet, C, Gouzy, et al. Regional assignment of genetic markers using a somatic cell hybrid panel: a WWW interactive program available for the pig genome Computer Application Bioscience, 1 (1997),pp. 69-73
[12]	Milan, D, Hawken, et al. IMpRH server: an RH mapping server available on the web Bioinformatics, 16 (2000),pp. 558-559
[13]	Yerle, M, Echard, et al. A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics Cytogenetics Cell Genetics, 73 (1996),pp. 194-202
[14]	Yerle, M, Pinton, et al. Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs Cytogenet Cell Genet, 82 (1998),pp. 182-188