5.9
CiteScore
5.9
Impact Factor

2016 Vol. 43, No. 12

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Original research
Zinc finger factor ZNF121 is a MYC-interacting protein functionally affecting MYC and cell proliferation in epithelial cells
Ang Luo, Xuan Zhang, Liya Fu, Zhengmao Zhu, Jin-Tang Dong
2016, 43(12): 677-685. doi: 10.1016/j.jgg.2016.05.006
Abstract (78) HTML PDF (1)
Abstract:
MYC is a potent oncoprotein that modulates multiple cellular processes including proliferation, apoptosis, differentiation, stemness, senescence, and migration. Functioning primarily as a transcriptional factor, MYC interacts with a large number of proteins, and identification and characterization of MYC-interacting proteins are important for understanding how MYC functions. In this study, we used different systems to demonstrate that a novel zinc finger transcription factor, ZNF121, physically interacted with MYC, and the interaction involved their N-terminal regions. Overexpression of ZNF121 increased, while its knockdown decreased, the expression of MYC in multiple epithelial cell lines, and MYC had similar effects on ZNF121 expression. An expression correlation was also detectable in a panel of epithelial cell lines and a cohort of human breast cancer tissues. Functionally, knockdown ofZNF121 in several breast epithelial cell lines attenuated the expressions of MYC and its target genes (e.g., EGR1, CDC2, and nucleolin) and slowed cell proliferation, accompanied by cell cycle arrest in the G1 phase and expression alteration of cell cycle regulators (cyclin D1, p14 and p21). Analysis of publically available databases showed that ZNF121 expression is up-regulated in human breast cancer, and the up-regulation significantly associates with worse patient survival in the luminal A subtype of breast cancer. These findings establish ZNF121 as a MYC-interacting protein with functional effects on MYC and cell proliferation.
BLOC-2 subunit HPS6 deficiency affects the tubulation and secretion of von Willebrand factor from mouse endothelial cells
Jing Ma, Zhe Zhang, Lin Yang, Janos Kriston-Vizi, Daniel F. Cutler, Wei Li
2016, 43(12): 686-693. doi: 10.1016/j.jgg.2016.09.007
Abstract (97) HTML PDF (1)
Abstract:
Hermansky-Pudlak syndrome (HPS) is a recessive disorder with bleeding diathesis, which has been linked to platelet granule defects. Both platelet granules and endothelial Weibel-Palade bodies (WPBs) are members of lysosome-related organelles (LROs) whose formation is regulated by HPS protein associated complexes such as BLOC (biogenesis of lysosome-related organelles complex) -1, -2, -3, AP-3 (adaptor protein complex-3) and HOPS (homotypic fusion and protein sorting complex). Von Willebrand factor (VWF) is critical to hemostasis, which is stored in a highly-multimerized form as tubules in the WPBs. In this study, we found the defective, but varying, release of VWF into plasma after desmopressin (DDAVP) stimulation in HPS1 (BLOC-3 subunit), HPS6 (BLOC-2 subunit), and HPS9 (BLOC-1 subunit) deficient mice. In particular, VWF tubulation, a critical step in VWF maturation, was impaired in HPS6 deficient WPBs. This likely reflects a defective endothelium, contributing to the bleeding tendency in HPS mice or patients. The differentially defective regulated release of VWF in these HPS mouse models suggests the need for precise HPS genotyping before DDAVP administration to HPS patients.
A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice
Rui Yang, Yafei Li, Yan Su, Yi Shen, Ding Tang, Qiong Luo, Zhukuan Cheng
2016, 43(12): 694-701. doi: 10.1016/j.jgg.2016.09.006
Abstract (118) HTML PDF (4)
Abstract:
An awned rice (Oryza sativa) plant carrying a tiny extra chromosome was discovered among the progeny of a telotrisomic line 2n+4L. Fluorescence in situ hybridization (FISH) using chromosome specific BAC clones revealed that this extra chromosome was a ring chromosome derived from part of the long arm of chromosome 4. So the aneuploidy plant was accordingly named as 2n+4L ring. We did not detect any CentO FISH signals on the ring chromosome, and found only the centromeric probe Centromeric Retrotransposon of Rice (CRR) was co-localized with the centromere-specific histone CENH3 as revealed by sequential FISH after immunodetection. The extra ring chromosome exhibited a unique segregation pattern during meiosis, including no pairing between the ring chromosome and normal chromosome 4 during prophase I and pre-separation of sister chromatids at anaphase I.
Letter to the editor
Large-scale genome-wide scans do not support petaloid toenail as a Mendelian trait
Manfei Zhang, Sijie Wu, Juan Zhang, Yajun Yang, Jingze Tan, Haijuan Guan, Yu Liu, Kun Tang, Jean Krutmann, Shuhua Xu, Li Jin, Yaqun Guan, Hui Li, Sijia Wang
2016, 43(12): 702-704. doi: 10.1016/j.jgg.2016.10.003
Abstract (91) HTML PDF (1)
Abstract:
A highly efficient method for enriching TALEN or CRISPR/Cas9-edited mutant cells
Hideyo Yasuda, Eunsu Kim, Abu Musa Md Talimur Reza, Jin-Hoi Kim
2016, 43(12): 705-708. doi: 10.1016/j.jgg.2016.10.006
Abstract (98) HTML PDF (2)
Abstract:
Tumor suppressor p53 functions as an essential antiviral molecule against Japanese encephalitis virus
Xufang Deng, Jianchao Wei, Zixue Shi, Wenjun Yan, Zhuanchang Wu, Donghua Shao, Beibei Li, Ke Liu, Xiaodu Wang, Yafeng Qiu, Zhiyong Ma
2016, 43(12): 709-712. doi: 10.1016/j.jgg.2016.07.002
Abstract (79) HTML PDF (1)
Abstract: